|ProductName||Human uPAR ELISA Kit|
|CustomsName||Human uPAR ELISA Kit|
|Assay Type||Sandwich ELISA|
|Suitable Sample Type||serum, plasma, cell culture supernates|
|Format||96-well strip plate|
|Storage||4℃ (unopened) standard stored at -20℃, others stored at 4℃ (opened)|
|Sample Volume||10 μl|
|Standard Curve Range||62.50 - 4000 pg/ml|
|Spike Recovery Range||87 % - 114%|
|Mean Spike Recovery||1.01|
|CV of Intra plate||3.0 % - 4.7 %|
|CV of Inter plate||2.1 % - 5.0 %|
|Components||96-well polystyrene microplate coated with a monoclonal antibody against uPAR|
Human uPAR Standard, lyophilized
uPAR Detect Antibody
Assay Buffer (10×)
Washing Buffer (20×)
|Describtion||This assay employs the quantitative sandwich enzyme immunoassay technique for the quantitative detection of human uPAR. The Human uPAR ELISA is for research use only. Not for diagnostic or therapeutic procedures.|
Urokinase-type Plasminogen Activator Receptor (uPAR) is a cell surface receptor that binds urokinase-type plasminogen activator (uPA) with high affinity, thereby facilitating the pericellular activation of plasminogen. It is multidomain glycoprotein tethered to the cell membrane with a glycosylphosphotidylinositol (GPI) anchor. uPAR was originally identified as a saturable binding site for urokinase on the cell surface.
uPAR binds urokinase and thus restricts plasminogen activation to the immediate vicinity of the cell membrane. Besides the primary ligand urokinase, uPAR interacts with several other proteins, among others: vitronectin, the uPAR associated protein and the integrin family of membrane proteins.
uPAR is a part of the plasminogen activation system, which in the healthy body is involved in tissue reorganization events such as mammary gland involution and wound healing. uPAR has been involved in various other processes related to cancer, such as cell migration, cell cycle regulation, and cell adhesion.