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Human UPAR Enzyme Immunoassay Kit

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Product Details

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ProductNameHuman uPAR ELISA Kit
CustomsNameHuman uPAR ELISA Kit
Assay TypeSandwich ELISA
Suitable Sample Typeserum, plasma, cell culture supernates
Format96-well strip plate
Storage4℃ (unopened) standard stored at -20℃, others stored at 4℃ (opened)
Shipping Condition4℃
Sample Volume10 μl
Sensitivity0.87 pg/ml
Standard Curve Range62.50 - 4000 pg/ml
Spike Recovery Range87 % - 114%
Mean Spike Recovery1.01
CV of Intra plate3.0 % - 4.7 %
CV of Inter plate2.1 % - 5.0 %
Components96-well polystyrene microplate coated with a monoclonal antibody against uPAR
Human uPAR Standard, lyophilized
uPAR Detect Antibody
Standard Diluent
Assay Buffer (10×)
Substrate (TMB)
Stop Solution
Washing Buffer (20×)
Plate Covers
DescribtionThis assay employs the quantitative sandwich enzyme immunoassay technique for the quantitative detection of human uPAR. The Human uPAR ELISA is for research use only. Not for diagnostic or therapeutic procedures.
Urokinase-type Plasminogen Activator Receptor (uPAR) is a cell surface receptor that binds urokinase-type plasminogen activator (uPA) with high affinity, thereby facilitating the pericellular activation of plasminogen. It is multidomain glycoprotein tethered to the cell membrane with a glycosylphosphotidylinositol (GPI) anchor. uPAR was originally identified as a saturable binding site for urokinase on the cell surface.
uPAR binds urokinase and thus restricts plasminogen activation to the immediate vicinity of the cell membrane. Besides the primary ligand urokinase, uPAR interacts with several other proteins, among others: vitronectin, the uPAR associated protein and the integrin family of membrane proteins.
uPAR is a part of the plasminogen activation system, which in the healthy body is involved in tissue reorganization events such as mammary gland involution and wound healing. uPAR has been involved in various other processes related to cancer, such as cell migration, cell cycle regulation, and cell adhesion.
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