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Human VEGF ELISA Kit DistrinButor

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Product Details

Standard - EK183.jpg


BrandMultiSciences
CatNum70-EK183
ProductNameHuman VEGF ELISA Kit
CustomsNameHuman VEGF ELISA Kit
ApplicationELISA
ReactivityHuman
Assay TypeSandwich ELISA
Suitable Sample Typeserum, plasma, cell culture supernates
Format96-well strip plate
Storage4℃ (unopened) standard stored at -20℃, others stored at 4℃ (opened)
Shipping Condition4℃
Sample Volume50 μl
Sensitivity3.66 pg/ml
Standard Curve Range31.25 - 2000 pg/ml
Spike Recovery Range95 % - 111 %
Mean Spike Recovery1.02
CV of Intra plate3.1 % - 3.5 %
CV of Inter plate3.2 % - 4.2 %
Components96-well polystyrene microplate coated with a monoclonal antibody against VEGF
Human VEGF Standard, lyophilized
VEGF Detect Antibody
Standard Diluent
Streptavidin-HRP
Assay Buffer (10×)
Substrate (TMB)
Stop Solution
washing Buffer (20×)
Plate Covers
DescribtionThis assay employs the quantitative sandwich enzyme immunoassay technique for the quantitative detection of human VEGF. The Human VEGF ELISA is for research use only. Not for diagnostic or therapeutic procedures.
Vascular endothelial growth factor (VEGF) is a sub-family of growth factors, to be specific, the platelet-derived growth factor family of cystine-knot growth factors. They are important signaling proteins involved in both vasculogenesis (the denovoformation of the embryonic circulatory system) and angiogenesis (the growth of blood vessels from pre-existing vasculature).VEGF is a signal protein produced by cells that imulates vasculogenesis and angiogenesis. It is part of the system that restores the oxygen supply to tissues when blood circulation is inadequate. VEGF is best known for its role in vasculogenesis. During embryogenesis, VEGF regulates the proliferation, migration, and survival of endothelial cells, thus regulating blood vessel density and size, but playing no role in determining vascular patterns.
Serum concentration of VEGF is high in bronchial asthma and diabetes mellitus. When VEGF is overexpressed, it can contribute to disease. Solid cancers cannot grow beyond a limited size without an adequate blood supply; cancers that can express VEGF are able to grow and metastasize. Overexpression of VEGF can cause vascular disease in the retina of the eye and other parts of the body. Drugs such as bevacizumab and Ranibizumab can inhibit VEGF and control or slow those diseases.

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