Human Vitronectin/VTN ELISA Kit Plate

Cat Num

70-EK1199

Product Name

Human Vitronectin/VTN ELISA Kit

Customs Name

Human Vitronectin/VTN ELISA Kit

Application

ELISA

Reactivity

Human

Assay Type

Sandwich ELISA

Suitable Sample Type

serum, plasma, cell culture supernates

Format

96-well strip plate

Storage

4℃ (unopened)standard stored at -20℃, others stored at 4℃ (opened)

Shipping Condition

4℃

Sample Volume

10 μl (prediluted)

Sensitivity

13.41 pg/ml

Standard Curve Range

250.00 -16000 pg/ml

Spike Recovery Range

-

Mean Spike Recovery

-

CV of Intra plate

3.9 % - 6.7 %

CV of Inter plate

2.6 % - 4.0 %

Components

96-well polystyrene microplate coated with a monoclonal antibody against VTN
Human VTN Standard, lyophilized
VTN Detect Antibody
Assay Buffer (10×)
Substrate (TMB)
Stop Solution
Washing Buffer (20×)
Plate Covers

Describtion

This assay employs the quantitative sandwich enzyme immunoassay technique for the quantitative detection of human VTN. The Human Vitronectin/VTN ELISA is for research use only. Not for diagnostic or therapeutic procedures.
Vitronectin (VTN), a member of the pexin family, is an abundant glycoprotein found in serum, the extracellular matrix and bone. It is a secreted protein and exists in either a single chain form or a clipped, two chain form held together by a disulfide bond. VTN consists of three domains: the N-terminal Somatomedin B domain, a central domain with hemopexin homology, and a C-terminal domain also with hemopexin homology.
VTN promotes cell adhesion and spreading, inhibits the membrane-damaging effect of the terminal cytolytic complement pathway, and binds to several serpins (serine protease inhibitors). It has been speculated to be involved in hemostasis, cell migration and tumor malignancy.