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RUO Human Granzyme K ELISA Kit Plate

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Product Details

Standard - EK164.jpg


BrandMultiSciences
CatNum70-EK164
ProductNameHuman Granzyme K ELISA Kit
CustomsNameHuman Granzyme K ELISA Kit
ApplicationELISA
ReactivityHuman
Assay TypeSandwich ELISA
Suitable Sample Typeserum, plasma, cell culture supernates
Format96-well strip plate
Storage4℃ (unopened) standard stored at -20℃, others stored at 4℃ (opened)
Shipping Condition4℃
Sample Volume20 μl
Sensitivity2.41 pg/ml
Standard Curve Range15.63 - 1000 pg/ml
Spike Recovery Range94 % - 107 %
Mean Spike Recovery1
CV of Intra plate5.5 % - 7.5 %
CV of Inter plate6.8 % - 16.4 %
Components96-well polystyrene microplate coated with a monoclonal antibody against Granzyme K
Human Granzyme K Standard, lyophilized
Granzyme K Detect Antibody
Standard Diluent
Streptavidin-HRP
Assay Buffer (10×)
Substrate (TMB)
Stop Solution
washing Buffer (20×)
Plate Covers
DescribtionThis assay employs the quantitative sandwich enzyme immunoassay technique for the quantitative detection of human Granzyme K. The Human Granzyme K ELISA is for research use only. Not for diagnostic or therapeutic procedures.
Granzymes are serine proteases that are released by cytoplasmic granules within cytotoxic T cells and natural killer cells. Their purpose is to induce apoptosis within virus-infected cells, thus destroying them. Granzymes include GranzymeA, Granzyme B, Granzyme H, Granzyme K. Granzyme K is a protein that in humans is encoded by the GZMK gene.This gene product is a member of a group of related serine proteases from the cytoplasmic granules of cytotoxic lymphocytes. Cytolytic T lymphocytes (CTL) and natural killer (NK) cells share the remarkable ability to recognize, bind, and lyse specific target cells. They are thought to protect their host by lysing cells bearing on their surface 'nonself' antigens, usually peptides or proteins resulting from infection by intracellular pathogens. The protein described here lacks consensus sequences for N-glycosylation present in other granzymes.
Granzyme K have been found in high levels of patients who have gone septic.
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