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Mouse Dkk-1 ELISA Kit Distributor

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Product Details

Standard - EK2111.jpg

BrandMultiSciences
CatNum70-EK2111
ProductNameMouse Dkk-1 ELISA Kit
CustomsNameMouse Dkk-1 ELISA Kit
ApplicationELISA
ReactivityMouse
Assay TypeSandwich ELISA
Suitable Sample Typeserum, plasma, cell culture supernates
Format96-well strip plate
Storage4℃ (unopened) standard stored at -20℃, others stored at 4℃ (opened)
Shipping Condition4℃
Sample Volume100 μl (prediluted)
Sensitivity3.14 pg/ml
Standard Curve Range93.75 - 6000 pg/ml
Spike Recovery Range80 % - 118 %
Mean Spike Recovery1
CV of Intra plate2.7 % - 4.2 %
CV of Inter plate2.5 % - 4.2 %
Components96-well polystyrene microplate coated with a monoclonal antibody against Dkk-1
Mouse Dkk-1 Standard, lyophilized
Dkk-1 Detect Antibody
Standard Diluent
Streptavidin-HRP
Assay Buffer (10×)
Substrate (TMB)
Stop Solution
Washing Buffer (20×)
Plate Covers
DescribtionThis assay employs the quantitative sandwich enzyme immunoassay technique for the quantitative detection of mouse Dkk-1. The Mouse Dkk-1 ELISA is for research use only. Not for diagnostic or therapeutic procedures.
Dickkopf related protein 1 (Dkk-1) is a secreted protein with two cysteine rich regions and is involved in embryonic development through its inhibition of the Wnt signaling pathway. All mice that were homozygous for the Dkk-1 knockout were dead at birth due to defects in the cranium and structures formed by the neural crest, such as failed development of eyes, olfactory placodes, frontonasal mass and mandibular processes, as well as incomplete development of the forebrain and midbrain and fusion of the digits of the forelimb.
Elevated levels of Dkk-1 in bone marrow, plasma and peripheral blood is associated with the presence of osteolytic bone lesions in patients with multiple myeloma.
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