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Mouse Endoglin/CD105 ELISA Assay Kit

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Product Details

Standard - EK2149.jpg

BrandMultiSciences
CatNum70-EK2149
ProductNameMouse Endoglin/CD105 ELISA Kit
CustomsNameMouse Endoglin/CD105 ELISA Kit
ApplicationELISA
ReactivityMouse
Assay TypeSandwich ELISA
Suitable Sample Typeserum, plasma, cell culture supernates
Format96-well strip plate
Storage4℃ (unopened) standard stored at -20℃, others stored at 4℃ (opened)
Shipping Condition4℃
Sample Volume20 μl
Sensitivity3.36 pg/ml
Standard Curve Range156.25 - 10000 pg/ml
Spike Recovery Range83 % - 117 %
Mean Spike Recovery0.99
CV of Intra plate2.0 % - 5.3 %
CV of Inter plate4.1 % - 6.0 %
Components96-well polystyrene microplate coated with a monoclonal antibody against TGF-β1
Mouse TGF-β1 Standard, lyophilized
TGF-β1 Detect Antibody
Streptavidin-HRP
Assay Buffer (10×)
Substrate (TMB)
Stop Solution
washing Buffer (20×)
HCl
NaOH
Plate Covers
DescribtionThis assay employs the quantitative sandwich enzyme immunoassay technique for the quantitative detection of mouse Endoglin. The Mouse Endoglin/CD105 ELISA is for research use only. Not for diagnostic or therapeutic procedures.
Endoglin also known as CD105 is a type I membrane glycoprotein located on cell surfaces and is part of the TGF-β receptor complex. It is involved in modulating a response to the binding of TGF-β1, TGF-β3, activin-A, BMP-2 and BMP-7. It has been postulated that Endoglin is involved in the cytoskeletal organization affecting cell morphology and migration. Endoglin has a role in the development of the cardiovascular system and in vascular remodeling. Its expression is regulated during heart development. Experimental mice without the Endoglin gene die due to cardiovascular abnormalities. In humans, Endoglin may be involved in the autosomal dominant disorder known as hereditary hemorrhagic telangiectasia (HHT) type 1. This condition leads to frequent nose bleeds, telangiectases on skin and mucosa and may cause arteriovenous malformations in different organs including brain, lung, and liver.
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