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Mouse Granzyme B ELISA Kit Distributor

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Product Details

Standard - EK2173.jpg

BrandMultiSciences
CatNum70-EK2173
ProductNameMouse Granzyme B ELISA Kit
CustomsNameMouse Granzyme B ELISA Kit
ApplicationELISA
ReactivityMouse
Assay TypeSandwich ELISA
Suitable Sample Typeserum, plasma, cell culture supernates
Format96-well strip plate
Storage4℃ (unopened) standard stored at -20℃, others stored at 4℃ (opened)
Shipping Condition4℃
Sample Volume10 μl
Sensitivity0.25 pg/ml
Standard Curve Range312.50 - 20000 pg/ml
Spike Recovery Range72% -129 %
Mean Spike Recovery1.03
CV of Intra plate4.6% - 5.8 %
CV of Inter plate1.7 % - 6.5 %
Components96-well polystyrene microplate coated with a monoclonal antibody against IL-1α
Rat IL-1α Standard, lyophilized
IL-1α Detect Antibody
Standard Diluent
Streptavidin-HRP
Assay Buffer (10×)
Substrate (TMB)
Stop Solution
washing Buffer (20×)
Plate Covers
DescribtionThis assay employs the quantitative sandwich enzyme immunoassay technique for the quantitative detection of mouse Granzyme B. The Mouse Granzyme B ELISA is for research use only. Not for diagnostic or therapeutic procedures.
Granzyme B is a serine protease most commonly found in the granules of cytotoxic lymphocytes (CTLs) and natural killer cells (NK cells). It is secreted by these cells along with the pore forming protein perforin to mediate apoptosis in target cells. Granzyme B has also more recently found to be produced by a wide range of non-cytotoxic cells ranging from basophils and mast cells to smooth muscle cells. Granzyme B has shown to be involved in inducing inflammation by stimulating cytokine release and is also involved in extracellular matrix remodelling. Elevated levels of granzyme B are also implicated in a number of autoimmune diseases, several skin diseases, and type 1 diabetes.
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