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Mouse IL-15/IL-15R Sandwich ELISA Kit

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Product Details

Standard - EK215R.jpg

BrandMultiSciences
CatNum70-EK215R
ProductNameMouse IL-15/IL-15R ELISA Kit
CustomsNameMouse IL-15/IL-15R ELISA Kit
ApplicationELISA
ReactivityMouse
Assay TypeSandwich ELISA
Suitable Sample Typeserum, plasma, cell culture supernates
Format96-well strip plate
Storage4℃ (unopened) standard stored at -20℃, others stored at 4℃ (opened)
Shipping Condition4℃
Sample Volume20 μl
Sensitivity0.65 pg/ml
Standard Curve Range7.81 - 500 pg/ml
Spike Recovery Range88 % - 112 %
Mean Spike Recovery1.01
CV of Intra plate2.4 % - 4.5 %
CV of Inter plate3.0 % - 6.2 %
Components96-well polystyrene microplate coated with a monoclonal antibody against IL-15/IL-15R
Mouse IL-15/IL-15R Standard, lyophilized
IL-15/IL-15R Detect Antibody
Standard Diluent
Streptavidin-HRP
Assay Buffer (10×)
Substrate (TMB)
Stop Solution
washing Buffer (20×)
Plate Covers
DescribtionThis assay employs the quantitative sandwich enzyme immunoassay technique for the quantitative detection of mouse IL-15/IL-15R. The Mouse IL-15/IL-15R ELISA is for research use only. Not for diagnostic or therapeutic procedures.
Interleukin-15 (IL-15) is a proinflammatory protein that has been shown to play a role in the activation of neutrophils, dendritic cells and macrophages, and is essential to the development and survival of NK cells and CD8 T-cells. IL-15 activates Jak/Stat pathways by signaling through a heterotrimeric receptor. Despite the expression of IL-15 mRNA in many cell types, the secreted protein is rarely detectable in biological samples. Recent research suggests that IL-15 is retained inside the cell and is only secreted in complex with its unique receptor, IL-15R alpha. The soluble IL-15/IL-15R complex is produced by mouse dendritic cells, fibroblasts, and macrophages, and demonstrates a 10-100-fold increase in agonistic activity over IL-15 alone.
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