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Mouse IL-17AF Enzyme Immunoassay Kit

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Product Details

Standard - EK2177.jpg

BrandMultiSciences
CatNum70-EK21772
ProductNameMouse IL-17AF ELISA Kit
CustomsNameMouse IL-17AF ELISA Kit
ApplicationELISA
ReactivityMouse
Assay TypeSandwich ELISA
Suitable Sample Typeserum, plasma, cell culture supernates
Format96-well strip plate
Storage4℃ (unopened) standard stored at -20℃, others stored at 4℃ (opened)
Shipping Condition4℃
Sample Volume20 μl
Sensitivity4.54 pg/ml
Standard Curve Range46.88 - 3000 pg/ml
Spike Recovery Range73 % - 125 %
Mean Spike Recovery1.1
CV of Intra plate4.4 % - 7.7 %
CV of Inter plate4.7 % - 6.8 %
Components96-well polystyrene microplate coated with a monoclonal antibody against IL-17AF
Mouse IL-17AF Standard, lyophilized
IL-17AF Detect Antibody
Standard Diluent
Streptavidin-HRP
Assay Buffer (10×)
Substrate (TMB)
Stop Solution
Washing Buffer (20×)
Plate Covers
DescribtionThis assay employs the quantitative sandwich enzyme immunoassay technique for the quantitative detection of mouse IL-17AF. The Mouse IL-17AF ELISA is for research use only. Not for diagnostic or therapeutic procedures.
IL-17 family members are glycoproteins secreted as dimers that induce local cytokine production and recruit granulocytes to sites of inflammation. IL-17 is induced by IL-15 and IL-23, mainly in activated CD4+ T cells distinct from Th1 or Th2 cells. Of the six IL-17 family members, IL-17A and IL-17F share the strongest homology (50% amino acid identity) and the two genes are located in the same chromosomal region. IL-17A and IL-17F have been observed in tissue samples from various autoimmune diseases, such as rheumatoid arthritis, multiple sclerosis, psoriasis, inflammatory bowel disease, and asthma.
IL-17A expression is associated with many inflammatory diseases in humans, such as rheumatoid arthritis, multiple sclerosis, asthma, systemic lupus erythematosus, and allograft rejection. Expression of IL-17F is also linked with human inflammatory diseases, including asthma.
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