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Mouse IL-22 Enzyme Immunoassay Kit

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Product Details

Standard - EK222.jpg

BrandMultiSciences
CatNum70-EK222
ProductNameMouse IL-22 ELISA Kit
CustomsNameMouse IL-22 ELISA Kit
ApplicationELISA
ReactivityMouse
Assay TypeSandwich ELISA
Suitable Sample Typeserum, plasma, cell culture supernates
Format96-well strip plate
Storage4℃ (unopened) standard stored at -20℃, others stored at 4℃ (opened)
Shipping Condition4℃
Sample Volume20 μl
Sensitivity0.74 pg/ml
Standard Curve Range15.63 - 1000 pg/ml
Spike Recovery Range85 % - 118 %
Mean Spike Recovery0.94
CV of Intra plate2.9 % - 7.9 %
CV of Inter plate3.1 % - 5.4 %
Components96-well polystyrene microplate coated with a monoclonal antibody against IL-22
Mouse IL-22 Standard, lyophilized
IL-22 Detect Antibody
Standard Diluent
Streptavidin-HRP
Assay Buffer (10×)
Substrate (TMB)
Stop Solution
washing Buffer (20×)
Plate Covers
DescribtionThis assay employs the quantitative sandwich enzyme immunoassay technique for the quantitative detection of mouse IL-22. The Mouse IL-22 ELISA is for research use only. Not for diagnostic or therapeutic procedures.
IL-22 is an α-helical cytokine. IL-22 binds to a heterodimeric cell surface receptor composed of IL-10R2 and IL-22R1 subunits.IL-22R is expressed on tissue cells, and it is absent on immune cells.In the mouse, the IL-22 gene is located on chromosome 10, in the same region as the IFN gamma gene. IL-22, in contrast to its relative IFN gamma, regulates the expression of only a few genes in keratinocytes. This is due to varied signal transduction. IL-22 biological activity is initiated by binding to a cell-surface complex composed of IL-22R1 and IL-10R2 receptor chains and further regulated by interactions with a soluble binding protein, IL-22BP, which shares sequence similarity with an extracellular region of IL-22R1 (sIL-22R1). IL-22 and IL-10 receptor chains play a role in cellular targeting and signal transduction to selectively initiate and regulate immune responses.
IL-22 can contribute to immune disease through the stimulation of inflammatory responses, S100s and defensins. IL-22 also promotes hepatocyte survival in the liver and epithelial cells in the lung and gut similar to IL-10.
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