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Mouse IL-23 ELISA Kit Distributor

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Product Details

Standard - EK223.jpg

BrandMultiSciences
CatNum70-EK223
ProductNameMouse IL-23 ELISA Kit
CustomsNameMouse IL-23 ELISA Kit
ApplicationELISA
ReactivityMouse
Assay TypeSandwich ELISA
Suitable Sample Typeserum, plasma, cell culture supernates
Format96-well strip plate
Storage4℃ (unopened) standard stored at -20℃, others stored at 4℃ (opened)
Shipping Condition4℃
Sample Volume20 μl
Sensitivity3.36 pg/ml
Standard Curve Range1.56 - 100 pg/ml
Spike Recovery Range83 % - 117 %
Mean Spike Recovery0.99
CV of Intra plate2.0 % - 5.3 %
CV of Inter plate4.1 % - 6.0 %
Components96-well polystyrene microplate coated with a monoclonal antibody against TGF-β1
Mouse TGF-β1 Standard, lyophilized
TGF-β1 Detect Antibody
Streptavidin-HRP
Assay Buffer (10×)
Substrate (TMB)
Stop Solution
washing Buffer (20×)
HCl
NaOH
Plate Covers
DescribtionThis assay employs the quantitative sandwich enzyme immunoassay technique for the quantitative detection of mouse IL-23. The Mouse IL-23 ELISA is for research use only. Not for diagnostic or therapeutic procedures.
IL-23 protein is a heterodimer, sharing a p40 subunit with IL-12, but having a distinct p19 subunit, which is IL-23 specific. Prior to the discovery of IL-23, IL-12 had been proposed to represent a key mediator of inflammation in mouse models of inflammation. However, many studies aimed at assessing the role of IL-12 had blocked the activity of IL-12p40, and were therefore not as specific as thought. Studies which blocked the function of IL-12p35 did not produce the same results as those targeting IL-12p40 as would have been expected if both subunits formed part of IL-12 only.
IL-23 was shown to be involved in inflammation of the nervous system and autoimmune diseases. IL-23 was shown to facilitate development of inflammation in numerous other models of immune pathology where IL-12 had previously been implicated including models of arthritis, intestinal inflammation, and psoriasis.
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