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Mouse IL-27 Cytokine ELISA Kit

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Product Details

Standard - EK227.jpg

BrandMultiSciences
CatNum70-EK227
ProductNameMouse IL-27 ELISA Kit
CustomsNameMouse IL-27 ELISA Kit
ApplicationELISA
ReactivityMouse
Assay TypeSandwich ELISA
Suitable Sample Typeserum, plasma, cell culture supernates
Format96-well strip plate
Storage4℃ (unopened) standard stored at -20℃, others stored at 4℃ (opened)
Shipping Condition4℃
Sample Volume20 μl
Sensitivity20.54 pg/ml
Standard Curve Range31.25 - 2000 pg/ml
Spike Recovery Range89 % - 113 %
Mean Spike Recovery1
CV of Intra plate3.3 % - 4.6 %
CV of Inter plate3.8 % - 4.1 %
Components96-well polystyrene microplate coated with a monoclonal antibody against CCL2
Mouse CCL2 Standard, lyophilized
CCL2 Detect Antibody
Standard Diluent
Streptavidin-HRP
Assay Buffer (10×)
Substrate (TMB)
Stop Solution
washing Buffer (20×)
Plate Covers
DescribtionThis assay employs the quantitative sandwich enzyme immunoassay technique for the quantitative detection of mouse IL-27. The Mouse IL-27 ELISA is for research use only. Not for diagnostic or therapeutic procedures.
IL-27, a member of the IL-12 family, is a heterodimeric protein consisting of the p40-related protein Epstein-Barr virus-induced gene 3 (EBI3) non-covalently linked to an IL-12p35-related protein, p28 (also known as IL-30). IL-27 plays an important function in regulating the activity of B- and T-lymphocytes. The effects of IL-27 are eliciting by its interaction with a specific cell-surface receptor complex composed of two proteins known as IL27R and gp130. Recent studies indicate that IL-27 has potent anti-tumor activity. It has been reported that IL-27 is critically involved in the induction of inflammation in rheumatoid arthritis.
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