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Mouse IL-33 Protein A ELISA Kit

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Product Details

Standard - EK233.jpg

ProductNameMouse IL-33 ELISA Kit
CustomsNameMouse IL-33 ELISA Kit
Assay TypeSandwich ELISA
Suitable Sample Typeserum, plasma, cell culture supernates
Format96-well strip plate
Storage4℃ (unopened) standard stored at -20℃, others stored at 4℃ (opened)
Shipping Condition4℃
Sample Volume20 μl
Sensitivity8.74 pg/ml
Standard Curve Range46.88 - 3000 pg/ml
Spike Recovery Range85 % - 117 %
Mean Spike Recovery0.98
CV of Intra plate4.9 % - 8.9 %
CV of Inter plate4.9 % - 8.1 %
Components96-well polystyrene microplate coated with a monoclonal antibody against IL-33
Mouse IL-33 Standard, lyophilized
IL-33 Detect Antibody
Standard Diluent
Assay Buffer (10×)
Substrate (TMB)
Stop Solution
washing Buffer (20×)
Plate Covers
DescribtionThis assay employs the quantitative sandwich enzyme immunoassay technique for the quantitative detection of mouse IL-33. The Mouse IL-33 ELISA is for research use only. Not for diagnostic or therapeutic procedures.
Interleukin 33 (IL-33), also known as NF-HEV and DVS 27, is a 30-kDa member of the IL-1 family produced by a wide variety of cell types, including fibroblasts, mast cells, dendritic cells, macrophages, osteoblasts, endothelial cells, and epithelial cells. IL-33 induces helper T cells, mast cells, eosinophils and basophils to produce type 2 cytokines. IL-33 mediates its biological effects by interacting with the receptors ST2 and IL-1 Receptor Accessory Protein (IL1RAP), activating intracellular molecules in the NF-κB and MAP kinase signaling pathways that drive production of type 2 cytokines (e.g. IL-5 and IL-13) from polarized Th2 cells. The induction of type 2 cytokines by IL-33 in vivo is believed to induce the severe pathological changes observed in mucosal organs following administration of IL-33.
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