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Mouse IL-9 ELISA Kit For Protein Quantification

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Product Details

Standard - EK209.jpg

BrandMultiSciences
CatNum70-EK209
ProductNameMouse IL-9 ELISA Kit
CustomsNameMouse IL-9 ELISA Kit
ApplicationELISA
ReactivityMouse
Assay TypeSandwich ELISA
Suitable Sample Typeserum, plasma, cell culture supernates
Format96-well strip plate
Storage4℃ (unopened) standard stored at -20℃, others stored at 4℃ (opened)
Shipping Condition4℃
Sample Volume20 μl
Sensitivity3.59 pg/ml
Standard Curve Range125.00 - 8000 pg/ml
Spike Recovery Range95 % - 117 %
Mean Spike Recovery1.08
CV of Intra plate2.5 % - 3.7 %
CV of Inter plate2.8 % - 4.6 %
Components96-well polystyrene microplate coated with a monoclonal antibody against IL-9
Mouse IL-9 Standard, lyophilized
IL-9 Detect Antibody
Standard Diluent
Streptavidin-HRP
Assay Buffer (10×)
Substrate (TMB)
Stop Solution
washing Buffer (20×)
Plate Covers
DescribtionThis assay employs the quantitative sandwich enzyme immunoassay technique for the quantitative detection of mouse IL-9. The Mouse IL-9 ELISA is for research use only. Not for diagnostic or therapeutic procedures.
Interleukin 9 (IL-9) is an extensively glycosylated protein of 14 kDa containing ten cysteine residues involved in disulfide bonding.The protein encoded by this gene is a cytokine produced by T-cells and specifically by CD4+ helper cells that acts as a regulator of a variety of hematopoietic cells. This cytokine stimulates cell proliferation and prevents apoptosis. It functions through the interleukin-9 receptor (IL-9R), which activates different signal transducer and activator (STAT) proteins and thus connects this cytokine to various biological processes. The gene encoding this cytokine has been identified as a candidate gene for asthma. Genetic studies on a mouse model of asthma demonstrated that this cytokine is a determining factor in the pathogenesis of bronchial hyperresponsiveness.
The existence of an IL-9-mediated autocrine loop has been suggested for some malignancies such as Hodgkin's disease and large cell anaplastic lymphoma for Hodgkin's cell lines. IL-9 is expressed by Reed-Sternberg cells and Hodgkin lymphoma cells and some large aplastic lymphoma cells, while non-Hodgkin lymphomas and peripheral T-cell lymphomas do not express it.

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