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Mouse LIF Sandwich ELISA Kit

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Product Details

Standard - EK2107.jpg

Factory NameMultisciences
CatNumEK2107-96
ProductNameMouse LIF ELISA Kit
CustomsNameMouse LIF ELISA Kit
ProductSpec96T
ApplicationELISA
ReactivityMouse
SDSSDS - EK2107
Assay TypeSandwich ELISA
Suitable Sample Typeserum, plasma, cell culture supernates
Format96-well strip plate
Storage4℃ (unopened) standard stored at -20℃, others stored at 4℃ (opened)
Shipping Condition4℃
Sample Volume50 μl (prediluted)
Sensitivity0.77 pg/ml
Standard Curve Range7.81 - 500 pg/ml
Spike Recovery Range91 % - 120%
Mean Spike Recovery108%
CV of Intra plate3.8 % - 4.8 %
CV of Inter plate3.9 % - 5.9 %
Components96-well polystyrene microplate coated with a monoclonal antibody against LIF
Mouse LIF Standard, lyophilized
LIF Detect Antibody
Standard Diluent
Streptavidin-HRP
Assay Buffer (10×)
Substrate (TMB)
Stop Solution
Washing Buffer (20×)
Plate Covers
DescribtionThis assay employs the quantitative sandwich enzyme immunoassay technique for the quantitative detection of mouse LIF. The Mouse LIF ELISA is for research use only. Not for diagnostic or therapeutic procedures.
Leukemia inhibitory factor (LIF) is an interleukin 6 family cytokine that affects cell growth by inhibiting differentiation. When LIF levels drop, the cells differentiate. Cells known to express LIF include activated T-cells, monocytes, and astrocytes, osteoblasts, keratinocytes, mast cells, and fibroblasts.
LIF derives its name from its ability to induce the terminal differentiation of myeloid leukemic cells, thus preventing their continued growth. Other properties attributed to the cytokine include: the growth promotion and cell differentiation of different types of target cells, influence on bone metabolism, cachexia, neural development, embryogenesis and inflammation. During pregnancy LIF is involved in decidualization of the maternal endometrium and implantation of the blastocyst to the endometrium. Women with decreased production of LIF and other cytokines are fertile and able to become pregnant, but there is an increased risk for unexplained, recurrent miscarriages. It has been suggested that recombinant human LIF might help to improve the implantation rate in women with unexplained infertility. In addition, LIF is typically added to stem cell culture medium to reduce spontaneous differentiation.
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