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Mouse MCPT-1/mMCP-1 ELISA Kit Plate

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Product Details

Standard - EK2176.jpg

BrandMultiSciences
CatNum70-EK2176
ProductNameMouse MCPT-1/mMCP-1 ELISA Kit
CustomsNameMouse MCPT-1/mMCP-1 ELISA Kit
ApplicationELISA
ReactivityMouse
Assay TypeSandwich ELISA
Suitable Sample Typeserum, plasma, cell culture supernates
Format96-well strip plate
Storage4℃ (unopened) standard stored at -20℃, others stored at 4℃ (opened)
Shipping Condition4℃
Sample Volume100 μl (prediluted)
Sensitivity44.56 pg/ml
Standard Curve Range62.50 - 4000 pg/ml
Spike Recovery Range80 % - 89 %
Mean Spike Recovery0.84
CV of Intra plate2.9 % - 3.9 %
CV of Inter plate1.4 % - 3.5 %
Components96-well polystyrene microplate coated with a monoclonal antibody against MCPT-1
Mouse MCPT-1 Standard, lyophilized
MCPT-1 Detect Antibody
Standard Diluent
Streptavidin-HRP
Assay Buffer (10×)
Substrate (TMB)
Stop Solution
Washing Buffer (20×)
Plate Covers
DescribtionThis assay employs the quantitative sandwich enzyme immunoassay technique for the quantitative detection of mouse MCPT-1. The Mouse MCPT-1/mMCP-1 ELISA is for research use only. Not for diagnostic or therapeutic procedures.
Mast cell protease-1 (MCPT-1), also known as β‑chymase, is a member of the Chymase family of chymotrypsin-like serine proteases and is the only chymase expressed by intestinal mucosal mast cells. Its activation is completed by the removal of a two residue N‑terminal propeptide by a dipeptidyl peptidase (Cathepsin C). Like human α‑Chymase, MCPT-1 is capable of the conversion of angiotensin I to angiotensin II, which plays a key role in the regulation of arterial pressure. MCPT-1 promotes mucosal permeability in intestinal allergic hypersensitivity reactions and plays an important role in host defense against intestinal parasites. Studies have shown that specific chymase inhibitors are able to diminish the development of abdominal aortic aneurysm and reduce the adhesion formation after cardiac surgery in hamsters. Therefore, the development of specific inhibitors of chymase activity has been a pharmacologic strategy to develop therapeutic agents.
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