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Mouse Myeloperoxidase/MPO Antibody ELISA Kit

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Product Details

Standard - EK2133.jpg

BrandMultiSciences
CatNum70-EK2133
ProductNameMouse myeloperoxidase/MPO ELISA Kit
CustomsNameMouse myeloperoxidase/MPO ELISA Kit
ApplicationELISA
ReactivityMouse
Assay TypeSandwich ELISA
Suitable Sample Typeserum, plasma, cell culture supernates
Format96-well strip plate
Storage4℃ (unopened) standard stored at -20℃, others stored at 4℃ (opened)
Shipping Condition4℃
Sample Volume20 μl (prediluted)
Sensitivity4.40 pg/ml
Standard Curve Range62.50 - 4000 pg/ml
Spike Recovery Range91 % - 107 %
Mean Spike Recovery0.99
CV of Intra plate3.8 % - 5.4 %
CV of Inter plate3.0 % - 4.1 %
Components96-well polystyrene microplate coated with a monoclonal antibody against MPO
Mouse MPO Standard, lyophilized
MPO Detect Antibody
Streptavidin-HRP
Assay Buffer (10×)
Substrate (TMB)
Stop Solution
Washing Buffer (20×)
Plate Covers
DescribtionThis assay employs the quantitative sandwich enzyme immunoassay technique for the quantitative detection of mouse MPO. The Mouse myeloperoxidase/MPO ELISA is for research use only. Not for diagnostic or therapeutic procedures.
Myeloperoxidase (MPO) is a peroxidase enzyme most abundantly expressed in neutrophil granulocytes, and produces hypohalous acids to carry out their antimicrobial activity. MPO oxidation of apoA-I reduces HDL-mediated inhibition of apoptosis and inflammation. MPO mediates protein nitrosylation and the formation of 3-chlorotyrosine and dityrosine crosslinks.
MPO deficiency is a hereditary deficiency of the enzyme, which predisposes to immune deficiency. Studies have reported an association between elevated MPO levels and the severity of coronary artery disease. It has also been suggested that MPO plays a significant role in the development of the atherosclerotic lesion and rendering plaques unstable.
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