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Mouse Osteopontin/OPN Cytokine ELISA Kit

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Product Details

Standard - EK2135.jpg


BrandMultiSciences
CatNum70-EK2135
ProductNameMouse osteopontin/OPN ELISA Kit
CustomsNameMouse osteopontin/OPN ELISA Kit
ApplicationELISA
ReactivityMouse
Assay TypeSandwich ELISA
Suitable Sample Typeserum, plasma, cell culture supernates
Format96-well strip plate
Storage4℃ (unopened) standard stored at -20℃, others stored at 4℃ (opened)
Shipping Condition4℃
Sample Volume10 μl (prediluted)
Sensitivity0.05 pg/ml
Standard Curve Range15.63 - 1000 pg/ml
Spike Recovery Range-
Mean Spike Recovery-
CV of Intra plate1.8 % - 3.4 %
CV of Inter plate2.1 % - 4.7 %
Components96-well polystyrene microplate coated with a monoclonal antibody against OPN
Mouse OPN Standard, lyophilized
OPN Detect Antibody
Streptavidin-HRP
Assay Buffer (10×)
Substrate (TMB)
Stop Solution
Washing Buffer (20×)
Plate Covers
DescribtionThis assay employs the quantitative sandwich enzyme immunoassay technique for the quantitative detection of mouse OPN. The Mouse osteopontin/OPN ELISA is for research use only. Not for diagnostic or therapeutic procedures.
Osteopontin (OPN) (also known as BSP-1, ETA-1, SPP-1 or Ric) encoded by the SPP1 gene is expressed mainly in bone. Regulation of the OPN gene is incompletely understood. Different cell types may differ in their regulatory mechanisms of the OPN gene. OPN has biological function in biomineralization, bone remodeling, immune functions, chemotaxis, cell activation and apoptosis, OPN also is associated with increased myocyte apoptosis and myocardial dysfunction.
OPN is associated with many physiological and pathological processes including wound healing, bone turnover, tumorigenesis, inflammation, ischemia, and immune responses. Manipulation of plasma (or local) OPN levels may be useful in the treatment of autoimmune diseases, cancer metastasis, bone (and tooth) mineralization diseases, osteoporosis, and some forms of stress.
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