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Mouse SICAM-1/CD54 Antibody ELISA Kit

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Product Details

Standard - EK289.jpg

BrandMultiSciences
CatNum70-EK289
ProductNameMouse sICAM-1/CD54 ELISA Kit
CustomsNameMouse sICAM-1/CD54 ELISA Kit
ApplicationELISA
ReactivityMouse
Assay TypeSandwich ELISA
Suitable Sample Typeserum, plasma, cell culture supernates
Format96-well strip plate
Storage4℃ (unopened) standard stored at -20℃, others stored at 4℃ (opened)
Shipping Condition4℃
Sample Volume100 μl Prediluted Sample
Sensitivity1.27 pg/ml
Standard Curve Range62.50 - 4000 pg/ml
Spike Recovery Range89 % - 112 %
Mean Spike Recovery1.02
CV of Intra plate3.6 % - 4.0 %
CV of Inter plate4.1 % - 5.1 %
Components96-well polystyrene microplate coated with a monoclonal antibody against sICAM-1
Mouse sICAM-1 Standard, lyophilized
sICAM-1 Detect Antibody
Streptavidin-HRP
Assay Buffer (10×)
Substrate (TMB)
Stop Solution
washing Buffer (20×)
Plate Covers
DescribtionThis assay employs the quantitative sandwich enzyme immunoassay technique for the quantitative detection of mouse sICAM-1. The Mouse sICAM-1/CD54 ELISA is for research use only. Not for diagnostic or therapeutic procedures.
Intercellular Adhesion Molecule 1 (ICAM-1), also known as CD54, is a nearly ubiquitous transmembrane glycoprotein which is typically expressed on endothelial cells and cells of the immune system and plays a key role in leukocyte migration and activation. It binds to integrins of type CD11a/CD18, or CD11b/CD18 and is also exploited by rhinovirus as a receptor. Soluble ICAM-1 (sICAM-1) promotes angiogenesis and serves as an indicator of vascular endothelial cell activation or damage. It also functions as an inhibitor of transmembrane ICAM-1 mediated activities such as monocyte adhesion to activated endothelial cells and sensitivity of tumor cells to NK cell-mediated lysis. Monitoring of sICAM-1 levels in serum provides more detailed insights in several pathological situations such as cardiovascular disease, type 2 diabetes, organ transplant dysfunction, oxidant stress, abdominal fat mass, hypertension, liver disease, and certain malignancies.
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