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Mouse TRANCE/TNFSF11/RANKL Antibody ELISA Kit

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Product Details

Standard - EK2208.jpg

BrandMultiSciences
CatNum70-EK2208
ProductNameMouse TRANCE/TNFSF11/RANKL ELISA Kit
CustomsNameMouse TRANCE/TNFSF11/RANKL ELISA Kit
ApplicationELISA
ReactivityMouse
Assay TypeSandwich ELISA
Suitable Sample Typeserum, plasma, cell culture supernates
Format96-well strip plate
Storage4℃ (unopened) standard stored at -20℃, others stored at 4℃ (opened)
Shipping Condition4℃
Sample Volume20 μl
Sensitivity0.74 pg/ml
Standard Curve Range31.25 - 2000 pg/ml
Spike Recovery Range85 % - 118 %
Mean Spike Recovery0.94
CV of Intra plate2.9 % - 7.9 %
CV of Inter plate3.1 % - 5.4 %
Components96-well polystyrene microplate coated with a monoclonal antibody against IL-22
Mouse IL-22 Standard, lyophilized
IL-22 Detect Antibody
Standard Diluent
Streptavidin-HRP
Assay Buffer (10×)
Substrate (TMB)
Stop Solution
washing Buffer (20×)
Plate Covers
DescribtionThis assay employs the quantitative sandwich enzyme immunoassay technique for the quantitative detection of mouse TRANCE. The Mouse TRANCE/TNFSF11/RANKL ELISA is for research use only. Not for diagnostic or therapeutic procedures.
TNF-related activation-induced cytokine (TRANCE), also known as tumor necrosis factor ligand superfamily member 11 (TNFSF11), receptor activator of nuclear factor kappa-B ligand (RANKL), is a member of the TNF superfamily. Cells known to express TRANCE include osteoblasts, CD4+ and CD8+ T cells, megakaryocytes, bronchial and intestinal epithelium and so on.
TRANCE appears to play a central role in both immune response and bone morphogenesis. During bone development and remodeling, TRANCE expression by osteoblasts in conjunction with M-CSF induces osteoclast formation and activation, resulting in bone resorption. This allows for subsequent bone deposition and remodeling. TRANCE also plays a role in pathological osteoporosis. Its inappropriate expression by activated lymphocytes and osteoblasts results in excessive joint and bone resorption vs. deposition. Overproduction of TRANCE is implicated in a variety of degenerative bone diseases, such as rheumatoid arthritis and psoriatic arthritis.
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