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Rat EGF ELISA Kit Distributor

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Product Details

Standard - EK393.jpg

BrandMultiSciences
CatNum70-EK393
ProductNameRat EGF ELISA Kit
CustomsNameRat EGF ELISA Kit
ApplicationELISA
ReactivityRat
Assay TypeSandwich ELISA
Suitable Sample Typeserum, plasma, cell culture supernates
Format96-well strip plate
Storage4℃ (unopened) standard stored at -20℃, others stored at 4℃ (opened)
Shipping Condition4℃
Sample Volume20 μl
Sensitivity0.33 pg/ml
Standard Curve Range15.63 - 1000 pg/ml
Spike Recovery Range94 % - 110 %
Mean Spike Recovery1.01
CV of Intra plate1.7 % - 5.0 %
CV of Inter plate5.9 % - 7.5 %
Components96-well polystyrene microplate coated with a monoclonal antibody against EGF
Rat EGF Standard, lyophilized
EGF Detect Antibody
Standard Diluent
Streptavidin-HRP
Assay Buffer (10×)
Substrate (TMB)
Stop Solution
Washing Buffer (20×)
Plate Covers
DescribtionThis assay employs the quantitative sandwich enzyme immunoassay technique for the quantitative detection of rat EGF. The Rat EGF ELISA is for research use only. Not for diagnostic or therapeutic procedures.
Epidermal growth factor (EGF) is a growth factor that stimulates cell growth, proliferation, and differentiation by binding to its receptor EGFR. EGF is a low-molecular-weight polypeptide first purified from the mouse submandibular gland, but since then found in many human tissues including submandibular gland, parotid gland. Salivary EGF, which seems also regulated by dietary inorganic iodine, also plays an important physiological role in the maintenance of oro-esophageal and gastric tissue integrity. The biological effects of salivary EGF include healing of oral and gastroesophageal ulcers, inhibition of gastric acid secretion, stimulation of DNA synthesis as well as mucosal protection from intraluminal injurious factors such as gastric acid, bile acids, pepsin, and trypsin and to physical, chemical and bacterial agents.Increased activity of the receptor for EGF has been observed in certain types of cancer, often correlated with mutations in the receptor and abnormal function such as constitutive receptor signalling independent of the levels of EGF or of binding of EGF.
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