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Rat IL-1α ELISA Kit Distributor

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Product Details

Standard - EK301A.jpg

BrandMultiSciences
CatNum70-EK301A
ProductNameRat IL-1α ELISA Kit
CustomsNameRat IL-1α ELISA Kit
ApplicationELISA
ReactivityRat
Assay TypeSandwich ELISA
Suitable Sample Typeserum, plasma, cell culture supernates
Format96-well strip plate
Storage4℃ (unopened) standard stored at -20℃, others stored at 4℃ (opened)
Shipping Condition4℃
Sample Volume20 μl
Sensitivity0.25 pg/ml
Standard Curve Range7.81 - 500 pg/ml
Spike Recovery Range72% -129 %
Mean Spike Recovery1.03
CV of Intra plate4.6% - 5.8 %
CV of Inter plate1.7 % - 6.5 %
Components96-well polystyrene microplate coated with a monoclonal antibody against IL-1α
Rat IL-1α Standard, lyophilized
IL-1α Detect Antibody
Standard Diluent
Streptavidin-HRP
Assay Buffer (10×)
Substrate (TMB)
Stop Solution
washing Buffer (20×)
Plate Covers
DescribtionThis assay employs the quantitative sandwich enzyme immunoassay technique for the quantitative detection of rat IL-1α. The Rat IL-1α ELISA is for research use only. Not for diagnostic or therapeutic procedures.
Interleukin-1 (IL-1) is an extracellular peptide of 17 kDa that designates two proteins, IL-1α and IL-1β. IL-1α is a pleiotropic cytokine that in humans is encoded by the IL1A gene. IL-1α is produced mainly by activated macrophages, as well as neutrophils, epithelial cells, and endothelial cells. It possesses metabolic, physiological, haematopoietic activities, and plays one of the central roles in the regulation of the immune responses.
IL-1α has been shown to interact with HAX1 and NDN, but the most clinically relevant is its synergism with TNF. IL-1α and TNF are both acute-phase cytokines that act to promote fever and inflammation.
IL-1α is currently being evaluated in clinical trials as a potential therapeutic in oncology indications. Blocking the activity of IL-1α has the potential to treat skin diseases such as acne.
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