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Rat IL-10 Protein A ELISA Kit

Welcome to buy the high quality and sanitary rat il-10 protein a elisa kit with our professional manufacturers and suppliers. We can assure of you of the high quality and competitive price of our ELISA kit for procedure.

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Product Details

Standard - EK310.jpg

BrandMultiSciences
CatNum70-EK310
Product NameRat IL-10 ELISA Kit
Customs NameRat IL-10 ELISA Kit
ApplicationELISA
ReactivityRat
Assay TypeSandwich ELISA
Suitable Sample Typeserum, plasma, cell culture supernates
Format96-well strip plate
Storage4℃ (unopened) standard stored at -20℃, others stored at 4℃ (opened)
Shipping Condition4℃
Sample Volume20 μl
Sensitivity0.48 pg/ml
Standard Curve Range62.50 - 4000 pg/ml
Spike Recovery Range86 % - 108 %
Mean Spike Recovery0.98
CV of Intra plate3.4 % - 5.3 %
CV of Inter plate4.5 % - 6.1 %
Release Date2016/8/20
Components96-well polystyrene microplate coated with a monoclonal antibody against IL-10
Rat IL-10 Standard, lyophilized
IL-10 Detect Antibody
Standard Diluent
Streptavidin-HRP
Assay Buffer (10×)
Substrate (TMB)
Stop Solution
Washing Buffer (20×)
Plate Covers
DescribtionThis assay employs the quantitative sandwich enzyme immunoassay technique for the quantitative detection of rat IL-10. The Rat IL-10 ELISA is for research use only. Not for use in therapeutic procedures.
Rat Interleukin 10 (IL-10) cDNA encodes a 178 amino acid (aa) residue precursor protein that contains an 18 aa signal sequence and a 160 aa mature protein. Mature rat IL-10 shares 85% and 74% aa sequence identity with mouse and human IL-10, respectively.
IL-10 is primarily produced by monocytes and, to a lesser extent, lymphocytes, namely type 2 T helper cells (Th2), mastocytes, CD4+ CD25+ Foxp3+ regulatory T cells, and in a certain subset of activated T cells and B cells. IL-10 is a cytokine with multiple, pleiotropic, effects in immunoregulation and inflammation. It downregulates the expression of Th1 cytokines, MHC class II antigens, and co-stimulatory molecules on macrophages. It also enhances B cell survival, proliferation, and antibody production. IL-10 can block NF-κB activity, and is involved in the regulation of the JAK-STAT signaling pathway.
The immunosuppressive properties of IL-10 suggest a possible clinical use of IL-10 in suppressing rejections of grafts after organ transplantations. IL-10 can furthermore exert strong anti-inflammatory activities.

Welcome to buy the high quality and sanitary rat il-10 protein a elisa kit with our professional manufacturers and suppliers. We can assure of you of the high quality and competitive price of our ELISA kit for procedure.

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