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Rat SICAM-1/CD54 ELISA Kit Plate

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Product Details

Standard - EK389.jpg

BrandMultiSciences
CatNum70-EK389
ProductNameRat sICAM-1/CD54 ELISA Kit
CustomsNameRat sICAM-1/CD54 ELISA Kit
ApplicationELISA
ReactivityRat
Assay TypeSandwich ELISA
Suitable Sample Typeserum, plasma, cell culture supernates
Format96-well strip plate
Storage4℃ (unopened) standard stored at -20℃, others stored at 4℃ (opened)
Shipping Condition4℃
Sample Volume100 μl (prediluted)
Sensitivity0.48 pg/ml
Standard Curve Range62.50 - 4000 pg/ml
Spike Recovery Range73 % - 90 %
Mean Spike Recovery0.83
CV of Intra plate3.8 % - 4.2 %
CV of Inter plate2.7 % - 3.4 %
Components96-well polystyrene microplate coated with a monoclonal antibody against sICAM-1
Rat sICAM-1 Standard, lyophilized
sICAM-1 Detect Antibody
Streptavidin-HRP
Assay Buffer (10×)
Substrate (TMB)
Stop Solution
Washing Buffer (20×)
Plate Covers
DescribtionThis assay employs the quantitative sandwich enzyme immunoassay technique for the quantitative detection of rat sICAM-1. The Rat sICAM-1/CD54 ELISA is for research use only. Not for diagnostic or therapeutic procedures.
Intercellular Adhesion Molecule 1 (ICAM-1), also known as CD54, is a nearly ubiquitous transmembrane glycoprotein which is typically expressed on endothelial cells and cells of the immune system and plays a key role in leukocyte migration and activation. It binds to integrins of type CD11a/CD18, or CD11b/CD18 and is also exploited by rhinovirus as a receptor. Soluble ICAM-1 (sICAM-1) promotes angiogenesis and serves as an indicator of vascular endothelial cell activation or damage. It also functions as an inhibitor of transmembrane ICAM-1 mediated activities such as monocyte adhesion to activated endothelial cells and sensitivity of tumor cells to NK cell-mediated lysis.
Monitoring of sICAM-1 levels in serum provides more detailed insights in several pathological situations such as cardiovascular disease, type 2 diabetes, organ transplant dysfunction, oxidant stress, abdominal fat mass, hypertension, liver disease, and certain malignancies.

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