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Rat TGF-Beta1 Assay ELISA Kit

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Product Details

Standard - EK381.jpg


BrandMultiSciences
CatNum70-EK381
ProductNameRat TGF-beta1 ELISA Kit
CustomsNameRat TGF-beta1 ELISA Kit
ApplicationELISA
ReactivityRat
Assay TypeSandwich ELISA
Suitable Sample Typeserum, plasma, cell culture supernates
Format96-well strip plate
Storage4℃ (unopened) standard stored at -20℃, others stored at 4℃ (opened)
Shipping Condition4℃
Sample Volume50 μl (prediluted)
Sensitivity3.36 pg/ml
Standard Curve Range31.25 - 2000 pg/ml
Spike Recovery Range83 % - 117 %
Mean Spike Recovery0.99
CV of Intra plate2.0 % - 5.3 %
CV of Inter plate4.1 % - 6.0 %
Components96-well polystyrene microplate coated with a monoclonal antibody against TGF-β1
Rat TGF-β1 Standard, lyophilized
TGF-β1 Detect Antibody
Streptavidin-HRP
Assay Buffer (10×)
Substrate (TMB)
Stop Solution
washing Buffer (20×)
HCl
NaOH
Plate Covers
DescribtionThis assay employs the quantitative sandwich enzyme immunoassay technique for the quantitative detection of rat TGF-β1. The Rat TGF-β1 ELISA is for research use only. Not for diagnostic or therapeutic procedures.
Transforming growth factor beta 1 (TGF-β1) is a polypeptide member of the transforming growth factor beta superfamily of cytokines that performs many cellular functions, including the control of cell growth,cell proliferation, cell differentiation and apoptosis. TGF-βs are a multifunctional set peptides that controlsproliferation, differentiation, and other functions in many cell types. TGF-βs act synergistically with TGFA in inducingtransformation. It also acts as a negative autocrine growth factor. Dysregulation of TGF-β activation and signaling may result in apoptosis. Many cells synthesize TGF-β and almost all of them have specific receptors for this peptide. TGF-β1, TGF-β2 , and TGF-β3 all function through the same receptor signaling systems. TGF-β1 plays an important role in controlling the immune system, and shows different activities on different types of cell, or cells at different developmental stages. Most immune cells (orleukocytes) secrete TGF-β1.
TGF-β1 is related to cancer, autoimmune diseases, liver diseases, kidney diseases, diabetes, cardiovascular diseases, asthma, chronic obstructive pulmonary disorder (COPD), cystic fibrosis (CF) and so on.

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