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Rat TNF-alpha Protein A ELISA Kit

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Product Details

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ProductNameRat TNF-alpha ELISA Kit
CustomsNameRat TNF-alpha ELISA Kit
Assay TypeSandwich ELISA
Suitable Sample Typeserum, plasma, cell culture supernates
Format96-well strip plate
Storage4℃ (unopened) standard stored at -20℃, others stored at 4℃ (opened)
Shipping Condition4℃
Sample Volume20 μl
Sensitivity7.14 pg/ml
Standard Curve Range78.13 - 5000 pg/ml
Spike Recovery Range82 % - 116 %
Mean Spike Recovery0.96
CV of Intra plate4.4 % - 6.3 %
CV of Inter plate6.0 % - 8.4 %
Components96-well polystyrene microplate coated with a monoclonal antibody against TNF-α
Rat TNF-α Standard, lyophilized
TNF-α Detect Antibody
Standard Diluent
Assay Buffer (10×)
Substrate (TMB)
Stop Solution
washing Buffer (20×)
Plate Covers
DescribtionThis assay employs the quantitative sandwich enzyme immunoassay technique for the quantitative detection of rat TNF-α. The Rat TNF-α ELISA is for research use only. Not for diagnostic or therapeutic procedures.
Tumor necrosis factor alpha (TNF-α), also known as cachectin and TNFSF1A, is an adipokine involved in systemic inflammation and is a member of a group of cytokines that stimulate the acute phase reaction. It is produced chiefly by activated macrophages, although it can be produced by many other cell types such as CD4+ lymphocytes, NK cells, neutrophils, mast cells, eosinophils, and neurons. It functions as a multipotent modulator of immune response and further acts as a potent pyrogen. TNF-α circulates throughout the body responding to stimuli (infectious agents or tissue injury), activating neutrophils, altering the properties of vascular endothelial cells, regulating metabolic activities of other tissues, as well as exhibiting tumoricidal activity by inducing localized blood clotting.
TNF-α may play a significant role in the pathogenesis of inflammatory disease of the joints and other tissues. Recently, a growing body of information has pointed to a role for TNF-α in the pathogenesis of AIDS. Measurement of TNF-α levels has also been shown to be useful in transplant research.
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