EasyGo!™ Human IL-10 One-Step ELISA Kit

$400.00$500.00

TARGETS IL10
SPECIES Human
Sample Type Serum, plasma, DMEM cell culture supernatant
Sample Volume Serum or plasma: 10μL; DMEM cell culture supernatant: 100μL
Sensitivity 11.71 pg/mL
Range 15.63 pg/mL – 1000 pg/mL
Assay Time 1.5 h
Recovery 90% – 107%
Average Recovery 0.91
Sample Type Serum, plasma, DMEM cell culture supernatant
Sample Volume Serum or plasma: 10μL; DMEM cell culture supernatant: 100μL
Sensitivity 11.71 pg/mL
Range 15.63 pg/mL – 1000 pg/mL
Assay Time 1.5 h
Recovery 90% – 107%
Average Recovery 0.91
Intra Precision 3.9% – 5.7%
Inter-Precision 4.5% – 7.3%
Platform ELISA
Plate Detachable 96-well plate
Size 96T/48T
Storage If the reagent kit is unopened, it should be stored at 4℃. However, if it has been opened, the standard solution should be stored at -20℃, while the other components should be stored at 4℃.
Delivery 4℃ blue ice transportation
Components 96-well polystyrene enzyme-linked immunosorbent assay (ELISA) plate coated with anti-IL10 monoclonal antibody
Human IL10 freeze-dried standard
IL10 detect Antibody
Standard Diluent
Assay Buffer(10×)
Substrate TMB
Stop Solution
Washing Buffer(20×)
Sealing Film
Assay Principle This assay employs the quantitative sandwich enzyme immunoassay technique. Amonoclonal antibody specific for human IL-10 has been immobilized onto microwells, and two pellets of the biotin-linked detect antibody specific for IL-10 (light yellow) and streptavidin-HRP (purple) are pre-placed in the microwells, sealed by the adhesive film. Standard or samples are pipetted intothe wells, then IL-10 present is bound by the immobilized antibody and detect antibody, of whichthe latter is conjugated with streptavidin-HRP in the incubation. After washing, substrate solutionreacts with HRP and color develops in proportion to the amount of IL-10 bound by the immobilized antibody. The color development is stopped and the intensity of the color is measuredby microplate reader

Targets

IL10

IL10 Target Infomation Overview

  • Target Symbol: IL10, interleukin 10
  • Gene Groups: Interleukins
  • Alias: CSIF; TGIF; IL10A; IL-10
  • Alias Names: cytokine synthesis inhibitory factor; T-cell growth inhibitory factor

IL10, interleukin 10 Target Infomation by Species

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Human IL10 Target Information

  • Target Symbol: IL10, interleukin 10
  • Alias:
    • CSIF
    • cytokine synthesis inhibitory factor
    • GVHDS
    • IL-10
    • IL10A
    • interleukin-10
    • MGC126450
    • MGC126451
    • T-cell growth inhibitory factor
    • TGIF
  • NCBI_Gene: 3586
  • UniProtKB: P22301

Human IL10 Predicted Functions

Enables protein dimerization activity. Involved in several processes, including negative regulation of cellular catabolic process; regulation of gene expression; and regulation of signal transduction. Acts upstream of or within several processes, including negative regulation of B cell proliferation; positive regulation of B cell apoptotic process; and positive regulation of signaling receptor activity. Located in extracellular space. Implicated in several diseases, including artery disease (multiple); autoimmune disease (multiple); eye disease (multiple); gastrointestinal system cancer (multiple); and hematologic cancer (multiple). Biomarker of several diseases, including autoimmune disease (multiple); diabetes mellitus (multiple); eye disease (multiple); female reproductive organ cancer (multiple); and hematologic cancer (multiple).

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Mouse Il10 Target Information

Mouse Il10 Predicted Functions

Enables cytokine activity. Involved in several processes, including negative regulation of cell death; negative regulation of heterotypic cell-cell adhesion; and negative regulation of vascular associated smooth muscle cell proliferation. Acts upstream of or within several processes, including cellular response to hepatocyte growth factor stimulus; negative regulation of cytokine production; and response to bacterium. Located in extracellular space. Is expressed in colon; liver; thymus; and thymus primordium. Used to study inflammatory bowel disease. Human ortholog(s) of this gene implicated in several diseases, including artery disease (multiple); autoimmune disease (multiple); eye disease (multiple); gastrointestinal system cancer (multiple); and hematologic cancer (multiple). Orthologous to human IL10 (interleukin 10).

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Rat Il10 Target Information

Rat Il10 Predicted Functions

Enables interleukin-10 receptor binding activity. Involved in several processes, including cellular response to estradiol stimulus; liver regeneration; and negative regulation of nitric oxide biosynthetic process. Located in extracellular space. Used to study several diseases, including lung disease (multiple); perinatal necrotizing enterocolitis; sciatic neuropathy; toxic encephalopathy; and transient cerebral ischemia. Biomarker of several diseases, including artery disease (multiple); decubitus ulcer; lung disease (multiple); obstructive sleep apnea; and postpartum depression. Human ortholog(s) of this gene implicated in several diseases, including artery disease (multiple); autoimmune disease (multiple); eye disease (multiple); gastrointestinal system cancer (multiple); and hematologic cancer (multiple). Orthologous to human IL10 (interleukin 10).

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IL10 Target News

[catlist tags=”il10″ template=targetnews thumbnail=yes thumbnail_class=”related-post-media clr” thumbnail_size=833]

13.3
Spatio-temporally controlled all-in-one hydrogel microspheres for ulcerative colitis treatment based on cocktail strategy
CHEMICAL ENGINEERING JOURNAL
1.4
Effects of continuous negative pressure suction combined with autologous platelet-rich gel on the levels of CRP, IL-6, wound healing and length of stay in clients with diabetic foot
TRANSFUSION AND APHERESIS SCIENCE
18.8
A citrullinated antigenic vaccine in treatment of autoimmune arthritis
Science Bulletin
4.6
Monocarboxylate Transporter 4 (Mct4) Facilitates Mycobacterium tuberculosis Survival through NF-κB p65 Mediated Interleukin-10 Production
iScience
2.3
Structure and biological activity in vitro of Flagellin and its mutants from Escherichia coli Nissle 1917
ARCHIVES OF MICROBIOLOGY
4.6
23-Hydroxybetulinic acid attenuates 5-fluorouracil resistance of colorectal cancer by modulating M2 macrophage polarization via STAT6 signaling
CANCER IMMUNOLOGY IMMUNOTHERAPY
13.3
A smart band-aid with temperature and reactive oxygen species dual-responsiveness for synergistic mechanical and pharmacological therapy of chronic wounds
CHEMICAL ENGINEERING JOURNAL
2.7
Poly(rC)-binding protein 1 alleviates neurotoxicity in 6-OHDA-induced SH-SY5Y cells and modulates glial cells in neuroinflammation
BRAIN RESEARCH
6.1
Solobacterium moorei promotes the progression of adenomatous polyps by causing inflammation and disrupting the intestinal barrier
Journal of Translational Medicine
3.6
Glycolysis Induced by METTL14 Is Essential for Macrophage Phagocytosis and Phenotype in Cervical Cancer
JOURNAL OF IMMUNOLOGY
5.7
IL4I1 in M2-like macrophage promotes glioma progression and is a promising target for immunotherapy
Frontiers in Immunology
12.4
Keratinocyte-to-macrophage communication exacerbate psoriasiform dermatitis via LRG1-enriched extracellular vesicles
Theranostics
3.2
Imbalance of follicular regulatory T (Tfr) cells/follicular helper T (Tfh) cells in adult patients with primary immune thrombocytopenia
EXPERIMENTAL BIOLOGY AND MEDICINE
2.8
Characteristics of Serum Autoantibody Repertoire and Immune Subgroup Variation of Tuberculosis-Associated Obstructive Pulmonary Disease
International Journal of Chronic Obstructive Pulmonary Disease
8
Single-cell transcriptomics reveals variations in monocytes and Tregs between gout flare and remission
JCI Insight
5.8
BMI-specific inflammatory response to phthalate exposure in early pregnancy: findings from the TMCHESC study
ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH
Allergic Reaction
Cardiovascular Disease (Hypertension & Atherosclerosis & Acute Myocardial Infarction & Coronary Heart Disease)
Eye Diseases (Glaucoma & Cataract)
Lung Disease (Asthma & COPD)
Multiple Sclerosis
Nervous System Diseases (Depression & Alzheimer & Epilepsy & Parkinson)
Obesity Diabetes
Osteoarthrosis & Rheumatoid Arthritis
Osteoporosis
Sepsis
Systemic Lupus Erythematosus
Transplantation
Tumor Marker

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