EasyGo!™ Human IL-10 One-Step ELISA Kit

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TARGETSIL10
SPECIESHuman
Sample TypeSerum, plasma, DMEM cell culture supernatant
Sample VolumeSerum or plasma: 10μL; DMEM cell culture supernatant: 100μL
Sensitivity11.71 pg/mL
Range15.63 pg/mL – 1000 pg/mL
Assay Time1.5 h
Recovery90% – 107%
Average Recovery0.91
Sample TypeSerum, plasma, DMEM cell culture supernatant
Sample VolumeSerum or plasma: 10μL; DMEM cell culture supernatant: 100μL
Sensitivity11.71 pg/mL
Range15.63 pg/mL – 1000 pg/mL
Assay Time1.5 h
Recovery90% – 107%
Average Recovery0.91
Intra Precision3.9% – 5.7%
Inter-Precision4.5% – 7.3%
PlatformELISA
PlateDetachable 96-well plate
Size96T/48T
StorageIf the reagent kit is unopened, it should be stored at 4℃. However, if it has been opened, the standard solution should be stored at -20℃, while the other components should be stored at 4℃.
Delivery4℃ blue ice transportation
Components96-well polystyrene enzyme-linked immunosorbent assay (ELISA) plate coated with anti-IL10 monoclonal antibody
Human IL10 freeze-dried standard
IL10 detect Antibody
Standard Diluent
Assay Buffer(10×)
Substrate TMB
Stop Solution
Washing Buffer(20×)
Sealing Film
Assay PrincipleThis assay employs the quantitative sandwich enzyme immunoassay technique. Amonoclonal antibody specific for human IL-10 has been immobilized onto microwells, and two pellets of the biotin-linked detect antibody specific for IL-10 (light yellow) and streptavidin-HRP (purple) are pre-placed in the microwells, sealed by the adhesive film. Standard or samples are pipetted intothe wells, then IL-10 present is bound by the immobilized antibody and detect antibody, of whichthe latter is conjugated with streptavidin-HRP in the incubation. After washing, substrate solutionreacts with HRP and color develops in proportion to the amount of IL-10 bound by the immobilized antibody. The color development is stopped and the intensity of the color is measuredby microplate reader

Targets

IL10

IL10 Target Infomation Overview

  • Target Symbol: IL10, interleukin 10
  • Gene Groups: Interleukins
  • Alias: CSIF; TGIF; IL10A; IL-10
  • Alias Names: cytokine synthesis inhibitory factor; T-cell growth inhibitory factor

IL10, interleukin 10 Target Infomation by Species

HumanMouseRat

Human IL10 Target Information

  • Target Symbol: IL10, interleukin 10
  • Alias:
    • CSIF
    • cytokine synthesis inhibitory factor
    • GVHDS
    • IL-10
    • IL10A
    • interleukin-10
    • MGC126450
    • MGC126451
    • T-cell growth inhibitory factor
    • TGIF
  • NCBI_Gene: 3586
  • UniProtKB: P22301

Human IL10 Predicted Functions

Enables protein dimerization activity. Involved in several processes, including negative regulation of cellular catabolic process; regulation of gene expression; and regulation of signal transduction. Acts upstream of or within several processes, including negative regulation of B cell proliferation; positive regulation of B cell apoptotic process; and positive regulation of signaling receptor activity. Located in extracellular space. Implicated in several diseases, including artery disease (multiple); autoimmune disease (multiple); eye disease (multiple); gastrointestinal system cancer (multiple); and hematologic cancer (multiple). Biomarker of several diseases, including autoimmune disease (multiple); diabetes mellitus (multiple); eye disease (multiple); female reproductive organ cancer (multiple); and hematologic cancer (multiple).

Mouse Il10 Target Information

Mouse Il10 Predicted Functions

Enables cytokine activity. Involved in several processes, including negative regulation of cell death; negative regulation of heterotypic cell-cell adhesion; and negative regulation of vascular associated smooth muscle cell proliferation. Acts upstream of or within several processes, including cellular response to hepatocyte growth factor stimulus; negative regulation of cytokine production; and response to bacterium. Located in extracellular space. Is expressed in colon; liver; thymus; and thymus primordium. Used to study inflammatory bowel disease. Human ortholog(s) of this gene implicated in several diseases, including artery disease (multiple); autoimmune disease (multiple); eye disease (multiple); gastrointestinal system cancer (multiple); and hematologic cancer (multiple). Orthologous to human IL10 (interleukin 10).

Rat Il10 Target Information

Rat Il10 Predicted Functions

Enables interleukin-10 receptor binding activity. Involved in several processes, including cellular response to estradiol stimulus; liver regeneration; and negative regulation of nitric oxide biosynthetic process. Located in extracellular space. Used to study several diseases, including lung disease (multiple); perinatal necrotizing enterocolitis; sciatic neuropathy; toxic encephalopathy; and transient cerebral ischemia. Biomarker of several diseases, including artery disease (multiple); decubitus ulcer; lung disease (multiple); obstructive sleep apnea; and postpartum depression. Human ortholog(s) of this gene implicated in several diseases, including artery disease (multiple); autoimmune disease (multiple); eye disease (multiple); gastrointestinal system cancer (multiple); and hematologic cancer (multiple). Orthologous to human IL10 (interleukin 10).

IL10 Target News

    Allergic Reaction
    Cardiovascular Disease (Hypertension & Atherosclerosis & Acute Myocardial Infarction & Coronary Heart Disease)
    Eye Diseases (Glaucoma & Cataract)
    Lung Disease (Asthma & COPD)
    Multiple Sclerosis
    Nervous System Diseases (Depression & Alzheimer & Epilepsy & Parkinson)
    Obesity Diabetes
    Osteoarthrosis & Rheumatoid Arthritis
    Osteoporosis
    Sepsis
    Systemic Lupus Erythematosus
    Transplantation
    Tumor Marker

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