Human Angiopoietin-1/ANG-1 ELISA Kit

$350.00$450.00

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TARGETSANGPT1
SPECIESHuman
Sample TypeSerum, plasma, cell culture supernatant, and other biological samples
Sample Volume50 μL
Sensitivity1.30 pg/mL
Range31.25 pg/mL – 2000 pg/mL
Assay Time3.5 h
Recovery80% – 110%
Average Recovery0.94
Sample TypeSerum, plasma, cell culture supernatant, and other biological samples
Sample Volume50 μL
Sensitivity1.30 pg/mL
Range31.25 pg/mL – 2000 pg/mL
Assay Time3.5 h
Recovery80% – 110%
Average Recovery0.94
Intra Precision3.3% – 5.4%
Inter-Precision5.0% – 8.2%
PlatformELISA
PlateDetachable 96-well plate
Size96T/48T
StorageIf the reagent kit is unopened, it should be stored at 4℃. However, if it has been opened, the standard solution should be stored at -20℃, while the other components should be stored at 4℃.
Delivery4℃ blue ice transportation
Components96-well polystyrene enzyme-linked immunosorbent assay (ELISA) plate coated with anti-Angipoietin-1/ANG-1 monoclonal antibody
Human Angipoietin-1/ANG-1 freeze-dried standard
Angipoietin-1/ANG-1 detect Antibody
Standard Diluent
Assay Buffer(10×)
Substrate TMB
Stop Solution
Washing Buffer(20×)
Sealing Film
Assay PrincipleThis kit utilizes the double antibody sandwich enzyme-linked immunosorbent assay (ELISA) detection technique.Specific anti-human ANG-1 antibodies are precoated on a high-affinity ELISA plate.Standards and test samples are added to the wells of the ELISA plate. After incubation, the ANG-1 present in the samples binds to the solid-phase antibodies. After washing to remove unbound substances, biotinylated detection antibodies are added and incubated. After washing to remove unbound biotinylated antibodies, streptavidin-HRP labeled with horseradish peroxidase is added. After washing, a colorimetric substrate, TMB, is added and the plate is incubated in the dark for color development. The intensity of the color reaction is directly proportional to the concentration of ANG-1 in the samples.A stop solution is added to terminate the reaction, and the absorbance value is measured at a wavelength of 450 nm (with a reference wavelength range of 570-630 nm).

 

Targets

ANGPT1

ANGPT1 Target Infomation Overview

  • Target Symbol: ANGPT1, angiopoietin 1
  • Gene Groups: Receptor ligands; Fibrinogen C domain containing
  • Alias: KIAA0003; Ang1

ANGPT1, angiopoietin 1 Target Infomation by Species

HumanMouseRat

Human ANGPT1 Target Information

Human ANGPT1 Predicted Functions

Enables receptor tyrosine kinase binding activity. Involved in several processes, including positive regulation of intracellular signal transduction; positive regulation of macromolecule metabolic process; and regulation of vascular permeability. Located in several cellular components, including extracellular space; membrane raft; and microvillus. Implicated in brain ischemia; diabetic retinopathy; and hypertension. Biomarker of carcinoma (multiple); hypertension; and ovarian cancer.

Mouse Angpt1 Target Information

Mouse Angpt1 Predicted Functions

Enables signaling receptor binding activity. Acts upstream of or within several processes, including blood vessel development; regulation of protein phosphorylation; and regulation of signal transduction. Predicted to be located in membrane raft; microvillus; and plasma membrane. Predicted to be active in collagen-containing extracellular matrix and extracellular space. Is expressed in several structures, including cardiovascular system; liver; lung; metanephros; and yolk sac. Human ortholog(s) of this gene implicated in brain ischemia; diabetic retinopathy; and hypertension. Orthologous to human ANGPT1 (angiopoietin 1).

Rat Angpt1 Target Information

Rat Angpt1 Predicted Functions

Enables identical protein binding activity. Involved in several processes, including animal organ development; response to estrogen; and response to vitamin B3. Located in extracellular space. Biomarker of gastric ulcer; in situ carcinoma; myocardial infarction; nephrosis; and pulmonary hypertension. Human ortholog(s) of this gene implicated in brain ischemia; diabetic retinopathy; and hypertension. Orthologous to human ANGPT1 (angiopoietin 1).

ANGPT1 Target News

    6.525
    System Analysis Based on Lipid-Metabolism-Related Genes Identifies AGT as a Novel Therapy Target for Gastric Cancer with Neoadjuvant Chemotherapy
    Pharmaceutics
    Sepsis

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