Human IGFBP-1 ELISA Kit

$350.00$450.00

Download:
TARGETSIGFBP1
SPECIESHuman
Sample TypeSerum, plasma, cell culture supernatant, and other biological samples
Sample Volume10 μL
Sensitivity0.93 pg/mL
Range31.25 pg/mL – 2000 pg/mL
Assay Time3.5 h
Recovery83% – 118%
Average Recovery1.01
Sample TypeSerum, plasma, cell culture supernatant, and other biological samples
Sample Volume10 μL
Sensitivity0.93 pg/mL
Range31.25 pg/mL – 2000 pg/mL
Assay Time3.5 h
Recovery83% – 118%
Average Recovery1.01
Intra Precision4.8% – 5.2%
Inter-Precision3.0% – 5.8%
PlatformELISA
PlateDetachable 96-well plate
Size96T/48T
StorageIf the reagent kit is unopened, it should be stored at 4℃. However, if it has been opened, the standard solution should be stored at -20℃, while the other components should be stored at 4℃.
Delivery4℃ blue ice transportation
Components96-well polystyrene enzyme-linked immunosorbent assay (ELISA) plate coated with anti-IGFBP-1 monoclonal antibody
Human IGFBP-1 freeze-dried standard
IGFBP-1 detect Antibody
Standard Diluent
HRP-labeled streptavidin
Assay Buffer(10×)
Substrate TMB
Stop Solution
Washing Buffer(20×)
Sealing Film
Assay PrincipleThis kit utilizes the double antibody sandwich enzyme-linked immunosorbent assay (ELISA) detection technique.Specific anti-human IGFBP-1 antibodies are precoated on a high-affinity ELISA plate.Standards and test samples are added to the wells of the ELISA plate. After incubation, the IGFBP-1 present in the samples binds to the solid-phase antibodies. After washing to remove unbound substances, biotinylated detection antibodies are added and incubated. After washing to remove unbound biotinylated antibodies, streptavidin-HRP labeled with horseradish peroxidase is added. After washing, a colorimetric substrate, TMB, is added and the plate is incubated in the dark for color development. The intensity of the color reaction is directly proportional to the concentration of IGFBP-1 in the samples.A stop solution is added to terminate the reaction, and the absorbance value is measured at a wavelength of 450 nm (with a reference wavelength range of 570-630 nm).

 

Targets

IGFBP1

IGFBP1 Target Infomation Overview

  • Target Symbol: IGFBP1, insulin like growth factor binding protein 1
  • Gene Groups: Insulin like growth factor binding proteins
  • Alias: IGF-BP25; AFBP; hIGFBP-1; PP12
  • Previous Names: IBP1
  • Alias Names: placental protein 12; amniotic fluid binding protein; alpha-pregnancy-associated endometrial globulin; growth hormone independent-binding protein; binding protein-28; binding protein-26; binding protein-25; IGF-binding protein 1; insulin-like growth factor binding protein 1

IGFBP1, insulin like growth factor binding protein 1 Target Infomation by Species

HumanMouseRat

Human IGFBP1 Target Information

  • Target Symbol: IGFBP1, insulin like growth factor binding protein 1
  • Alias:
    • AFBP
    • alpha-pregnancy-associated endometrial globulin
    • amniotic fluid binding protein
    • binding protein-25
    • binding protein-26
    • binding protein-28
    • growth hormone independent-binding protein
    • hIGFBP-1
    • IBP-1
    • IBP1
    • IGF-binding protein 1
    • IGF-BP25
    • IGFBP-1
    • insulin-like growth factor binding protein 1
    • insulin-like growth factor-binding protein 1
    • placental protein 12
    • PP12
  • NCBI_Gene: 3484
  • UniProtKB: P08833

Human IGFBP1 Predicted Functions

Enables insulin-like growth factor I binding activity and insulin-like growth factor II binding activity. Predicted to be involved in regulation of insulin-like growth factor receptor signaling pathway. Located in Golgi apparatus. Implicated in diabetic angiopathy. Biomarker of breast cancer.

Mouse Igfbp1 Target Information

Mouse Igfbp1 Predicted Functions

Enables insulin-like growth factor binding activity. Predicted to be involved in regulation of insulin-like growth factor receptor signaling pathway. Located in extracellular space. Is expressed in several structures, including blastocyst; early embryo; liver; and secondary oocyte. Human ortholog(s) of this gene implicated in diabetic angiopathy. Orthologous to human IGFBP1 (insulin like growth factor binding protein 1).

Rat Igfbp1 Target Information

  • Target Symbol: Igfbp1, insulin-like growth factor binding protein 1
  • Alias:
    • IBP-1
    • IBP1
    • IGF-binding protein 1
    • IGF-BP25
    • IGFBA
    • IGFBP-1
    • insulin-like growth factor-binding protein 1
    • placental protein 12
  • NCBI_Gene: 25685
  • UniProtKB: P21743

Rat Igfbp1 Predicted Functions

Predicted to enable insulin-like growth factor I binding activity and insulin-like growth factor II binding activity. Involved in several processes, including insulin receptor signaling pathway; positive regulation of cell growth; and tissue regeneration. Located in extracellular space. Human ortholog(s) of this gene implicated in diabetic angiopathy. Orthologous to human IGFBP1 (insulin like growth factor binding protein 1).

IGFBP1 Target News

    Obesity Diabetes

    Related Products

    more +

    EK1392

    $350.00$450.00

    EK1242

    $350.00$450.00

    EK1341

    $350.00$450.00

    EK1C01

    $350.00$450.00

    EK1334

    $350.00$450.00

    EK2295

    $350.00$450.00

    EK1360

    $350.00$450.00

    EK2100

    $350.00$450.00

    $350.00$450.00

    EK1349

    $350.00$450.00

    $350.00$450.00

    $350.00$450.00

    EK2153

    $350.00$450.00

    $350.00$450.00

    EK2F01

    $350.00$450.00

    EK2313

    $350.00$450.00

    Same Target