Human IGFBP-1 ELISA Kit

$350.00$450.00

TARGETS IGFBP1
SPECIES Human
Sample Type Serum, plasma, cell culture supernatant, and other biological samples
Sample Volume 10 μL
Sensitivity 0.93 pg/mL
Range 31.25 pg/mL – 2000 pg/mL
Assay Time 3.5 h
Recovery 83% – 118%
Average Recovery 1.01
Sample Type Serum, plasma, cell culture supernatant, and other biological samples
Sample Volume 10 μL
Sensitivity 0.93 pg/mL
Range 31.25 pg/mL – 2000 pg/mL
Assay Time 3.5 h
Recovery 83% – 118%
Average Recovery 1.01
Intra Precision 4.8% – 5.2%
Inter-Precision 3.0% – 5.8%
Platform ELISA
Plate Detachable 96-well plate
Size 96T/48T
Storage If the reagent kit is unopened, it should be stored at 4℃. However, if it has been opened, the standard solution should be stored at -20℃, while the other components should be stored at 4℃.
Delivery 4℃ blue ice transportation
Components 96-well polystyrene enzyme-linked immunosorbent assay (ELISA) plate coated with anti-IGFBP-1 monoclonal antibody
Human IGFBP-1 freeze-dried standard
IGFBP-1 detect Antibody
Standard Diluent
HRP-labeled streptavidin
Assay Buffer(10×)
Substrate TMB
Stop Solution
Washing Buffer(20×)
Sealing Film
Assay Principle This kit utilizes the double antibody sandwich enzyme-linked immunosorbent assay (ELISA) detection technique.Specific anti-human IGFBP-1 antibodies are precoated on a high-affinity ELISA plate.Standards and test samples are added to the wells of the ELISA plate. After incubation, the IGFBP-1 present in the samples binds to the solid-phase antibodies. After washing to remove unbound substances, biotinylated detection antibodies are added and incubated. After washing to remove unbound biotinylated antibodies, streptavidin-HRP labeled with horseradish peroxidase is added. After washing, a colorimetric substrate, TMB, is added and the plate is incubated in the dark for color development. The intensity of the color reaction is directly proportional to the concentration of IGFBP-1 in the samples.A stop solution is added to terminate the reaction, and the absorbance value is measured at a wavelength of 450 nm (with a reference wavelength range of 570-630 nm).

 

Targets

IGFBP1

IGFBP1 Target Infomation Overview

  • Target Symbol: IGFBP1, insulin like growth factor binding protein 1
  • Gene Groups: Insulin like growth factor binding proteins
  • Alias: IGF-BP25; AFBP; hIGFBP-1; PP12
  • Previous Names: IBP1
  • Alias Names: placental protein 12; amniotic fluid binding protein; alpha-pregnancy-associated endometrial globulin; growth hormone independent-binding protein; binding protein-28; binding protein-26; binding protein-25; IGF-binding protein 1; insulin-like growth factor binding protein 1

IGFBP1, insulin like growth factor binding protein 1 Target Infomation by Species

[su_tabs][su_tab title=”Human” disabled=”no” anchor=”” url=”” target=”blank” class=”tab-human”]

Human IGFBP1 Target Information

  • Target Symbol: IGFBP1, insulin like growth factor binding protein 1
  • Alias:
    • AFBP
    • alpha-pregnancy-associated endometrial globulin
    • amniotic fluid binding protein
    • binding protein-25
    • binding protein-26
    • binding protein-28
    • growth hormone independent-binding protein
    • hIGFBP-1
    • IBP-1
    • IBP1
    • IGF-binding protein 1
    • IGF-BP25
    • IGFBP-1
    • insulin-like growth factor binding protein 1
    • insulin-like growth factor-binding protein 1
    • placental protein 12
    • PP12
  • NCBI_Gene: 3484
  • UniProtKB: P08833

Human IGFBP1 Predicted Functions

Enables insulin-like growth factor I binding activity and insulin-like growth factor II binding activity. Predicted to be involved in regulation of insulin-like growth factor receptor signaling pathway. Located in Golgi apparatus. Implicated in diabetic angiopathy. Biomarker of breast cancer.

[/su_tab]
[su_tab title=”Mouse” disabled=”no” anchor=”” url=”” target=”blank” class=”tab-mouse”]

Mouse Igfbp1 Target Information

Mouse Igfbp1 Predicted Functions

Enables insulin-like growth factor binding activity. Predicted to be involved in regulation of insulin-like growth factor receptor signaling pathway. Located in extracellular space. Is expressed in several structures, including blastocyst; early embryo; liver; and secondary oocyte. Human ortholog(s) of this gene implicated in diabetic angiopathy. Orthologous to human IGFBP1 (insulin like growth factor binding protein 1).

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[su_tab title=”Rat” disabled=”no” anchor=”” url=”” target=”blank” class=”tab-rat”]

Rat Igfbp1 Target Information

  • Target Symbol: Igfbp1, insulin-like growth factor binding protein 1
  • Alias:
    • IBP-1
    • IBP1
    • IGF-binding protein 1
    • IGF-BP25
    • IGFBA
    • IGFBP-1
    • insulin-like growth factor-binding protein 1
    • placental protein 12
  • NCBI_Gene: 25685
  • UniProtKB: P21743

Rat Igfbp1 Predicted Functions

Predicted to enable insulin-like growth factor I binding activity and insulin-like growth factor II binding activity. Involved in several processes, including insulin receptor signaling pathway; positive regulation of cell growth; and tissue regeneration. Located in extracellular space. Human ortholog(s) of this gene implicated in diabetic angiopathy. Orthologous to human IGFBP1 (insulin like growth factor binding protein 1).

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IGFBP1 Target News

[catlist tags=”igfbp1″ template=targetnews thumbnail=yes thumbnail_class=”related-post-media clr” thumbnail_size=833]

4.2
Aberrant TCF21 upregulation in adenomyosis impairs endometrial decidualization by increasing PDE4C expression
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR BASIS OF DISEASE
7.9
The involvement of RNA N6‐methyladenosine and histone methylation modification in decidualization and endometriosis‐associated infertility
Clinical and Translational Medicine
Obesity Diabetes

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