Product Category
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Human IGFBP-1 ELISA Kit
$350.00 – $450.00
| Sample Type | Serum, plasma, cell culture supernatant, and other biological samples |
|---|---|
| Sample Volume | 10 μL |
| Sensitivity | 0.93 pg/mL |
| Range | 31.25 pg/mL – 2000 pg/mL |
| Assay Time | 3.5 h |
| Recovery | 83% – 118% |
| Average Recovery | 1.01 |
| Intra Precision | 4.8% – 5.2% |
| Inter-Precision | 3.0% – 5.8% |
| Platform | ELISA |
| Plate | Detachable 96-well plate |
| Size | 96T/48T |
| Storage | If the reagent kit is unopened, it should be stored at 4℃. However, if it has been opened, the standard solution should be stored at -20℃, while the other components should be stored at 4℃. |
| Delivery | 4℃ blue ice transportation |
| Components | 96-well polystyrene enzyme-linked immunosorbent assay (ELISA) plate coated with anti-IGFBP-1 monoclonal antibody Human IGFBP-1 freeze-dried standard IGFBP-1 detect Antibody Standard Diluent HRP-labeled streptavidin Assay Buffer(10×) Substrate TMB Stop Solution Washing Buffer(20×) Sealing Film |
| Assay Principle | This kit utilizes the double antibody sandwich enzyme-linked immunosorbent assay (ELISA) detection technique.Specific anti-human IGFBP-1 antibodies are precoated on a high-affinity ELISA plate.Standards and test samples are added to the wells of the ELISA plate. After incubation, the IGFBP-1 present in the samples binds to the solid-phase antibodies. After washing to remove unbound substances, biotinylated detection antibodies are added and incubated. After washing to remove unbound biotinylated antibodies, streptavidin-HRP labeled with horseradish peroxidase is added. After washing, a colorimetric substrate, TMB, is added and the plate is incubated in the dark for color development. The intensity of the color reaction is directly proportional to the concentration of IGFBP-1 in the samples.A stop solution is added to terminate the reaction, and the absorbance value is measured at a wavelength of 450 nm (with a reference wavelength range of 570-630 nm). |
Targets
IGFBP1
IGFBP1 Target Infomation Overview
- Target Symbol: IGFBP1, insulin like growth factor binding protein 1
- Gene Groups: Insulin like growth factor binding proteins
- Alias: IGF-BP25; AFBP; hIGFBP-1; PP12
- Previous Names: IBP1
- Alias Names: placental protein 12; amniotic fluid binding protein; alpha-pregnancy-associated endometrial globulin; growth hormone independent-binding protein; binding protein-28; binding protein-26; binding protein-25; IGF-binding protein 1; insulin-like growth factor binding protein 1
IGFBP1, insulin like growth factor binding protein 1 Target Infomation by Species
HumanMouseRat
Human IGFBP1 Target Information
- Target Symbol: IGFBP1, insulin like growth factor binding protein 1
- Alias:
- AFBP
- alpha-pregnancy-associated endometrial globulin
- amniotic fluid binding protein
- binding protein-25
- binding protein-26
- binding protein-28
- growth hormone independent-binding protein
- hIGFBP-1
- IBP-1
- IBP1
- IGF-binding protein 1
- IGF-BP25
- IGFBP-1
- insulin-like growth factor binding protein 1
- insulin-like growth factor-binding protein 1
- placental protein 12
- PP12
- NCBI_Gene: 3484
- UniProtKB: P08833
Human IGFBP1 Predicted Functions
Enables insulin-like growth factor I binding activity and insulin-like growth factor II binding activity. Predicted to be involved in regulation of insulin-like growth factor receptor signaling pathway. Located in Golgi apparatus. Implicated in diabetic angiopathy. Biomarker of breast cancer.
Mouse Igfbp1 Target Information
- Target Symbol: Igfbp1, insulin-like growth factor binding protein 1
- Alias:
- IGFBP-1
- NCBI_Gene: 16006
Mouse Igfbp1 Predicted Functions
Enables insulin-like growth factor binding activity. Predicted to be involved in regulation of insulin-like growth factor receptor signaling pathway. Located in extracellular space. Is expressed in several structures, including blastocyst; early embryo; liver; and secondary oocyte. Human ortholog(s) of this gene implicated in diabetic angiopathy. Orthologous to human IGFBP1 (insulin like growth factor binding protein 1).
Rat Igfbp1 Target Information
- Target Symbol: Igfbp1, insulin-like growth factor binding protein 1
- Alias:
- IBP-1
- IBP1
- IGF-binding protein 1
- IGF-BP25
- IGFBA
- IGFBP-1
- insulin-like growth factor-binding protein 1
- placental protein 12
- NCBI_Gene: 25685
- UniProtKB: P21743
Rat Igfbp1 Predicted Functions
Predicted to enable insulin-like growth factor I binding activity and insulin-like growth factor II binding activity. Involved in several processes, including insulin receptor signaling pathway; positive regulation of cell growth; and tissue regeneration. Located in extracellular space. Human ortholog(s) of this gene implicated in diabetic angiopathy. Orthologous to human IGFBP1 (insulin like growth factor binding protein 1).
