Human PDGFRB/CD140b ELISA Kit

$350.00$450.00

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TARGETSPDGFRB
SPECIESHuman
Sample TypeSerum, plasma, cell culture supernatant, and other biological samples
Sample Volume80 μL 1×Assay Buffer and 20 μL sample
Sensitivity48.54 pg/mL
Range0.63 ng/mL – 40 ng/mL
Assay Time3.5 h
Recovery70% – 117%
Average Recovery0.88
Sample TypeSerum, plasma, cell culture supernatant, and other biological samples
Sample Volume80 μL 1×Assay Buffer and 20 μL sample
Sensitivity48.54 pg/mL
Range0.63 ng/mL – 40 ng/mL
Assay Time3.5 h
Recovery70% – 117%
Average Recovery0.88
Intra Precision6.1% – 8.7%
Inter-Precision7.0%-8.2%
PlatformELISA
PlateDetachable 96-well plate
Size96T/48T
StorageIf the reagent kit is unopened, it should be stored at 4℃. However, if it has been opened, the standard solution should be stored at -20℃, while the other components should be stored at 4℃.
Delivery4℃ blue ice transportation
Components96-well polystyrene enzyme-linked immunosorbent assay (ELISA) plate coated with anti-PDGFRB/CD140b monoclonal antibody
Human PDGFRB/CD140b freeze-dried standard
PDGFRB/CD140b detect Antibody
Standard Diluent
Assay Buffer(10×)
Substrate TMB
Stop Solution
Washing Buffer(20×)
Sealing Film
Assay PrincipleThis kit utilizes the double antibody sandwich enzyme-linked immunosorbent assay (ELISA) detection technique.Specific anti-human PDGFRB antibodies are precoated on a high-affinity ELISA plate. Standard samples, test samples, and the detection antibody labeled with horseradish peroxidase are added to the wells of the ELISA plate. After incubation, PDGFRB present in the samples binds to the solid-phase antibody and the detection antibody. After washing, a colorimetric substrate, TMB, is added and the plate is incubated in the dark for color development. The intensity of the color reaction is directly proportional to the concentration of PDGFRB in the samples.A stop solution is added to terminate the reaction, and the absorbance value is measured at a wavelength of 450 nm (with a reference wavelength range of 570-630 nm).

 

Targets

PDGFRB

PDGFRB Target Infomation Overview

  • Target Symbol: PDGFRB, platelet derived growth factor receptor beta
  • Gene Groups: Receptor tyrosine kinases; CD molecules; I-set domain containing
  • Alias: JTK12; CD140b; PDGFR1
  • Previous Names: PDGFR
  • Alias Names: platelet-derived growth factor receptor, beta polypeptide

PDGFRB, platelet derived growth factor receptor beta Target Infomation by Species

HumanMouseRat

Human PDGFRB Target Information

  • Target Symbol: PDGFRB, platelet derived growth factor receptor beta
  • Alias:
    • Activated tyrosine kinase PDGFRB
    • beta-type platelet-derived growth factor receptor
    • CD140 antigen-like family member B
    • CD140B
    • IBGC4
    • IMF1
    • JTK12
    • KOGS
    • NDEL1-PDGFRB
    • PDGF-R-beta
    • PDGFR
    • PDGFR-1
    • PDGFR-beta
    • PDGFR1
    • PENTT
    • platelet-derived growth factor receptor 1
    • platelet-derived growth factor receptor beta
    • platelet-derived growth factor receptor, beta polypeptide
  • NCBI_Gene: 5159
  • UniProtKB: P09619

Human PDGFRB Predicted Functions

Enables several functions, including growth factor binding activity; platelet-derived growth factor beta-receptor activity; and platelet-derived growth factor receptor binding activity. Involved in several processes, including platelet-derived growth factor receptor signaling pathway; positive regulation of phosphate metabolic process; and protein phosphorylation. Located in Golgi apparatus and membrane. Is intrinsic component of plasma membrane. Implicated in basal ganglia calcification; glioblastoma; infantile myofibromatosis; myeloproliferative neoplasm (multiple); and renal cell carcinoma. Biomarker of Kuhnt-Junius degeneration; breast cancer (multiple); renal cell carcinoma; and reproductive organ cancer (multiple).

Mouse Pdgfrb Target Information

Mouse Pdgfrb Predicted Functions

Enables platelet-derived growth factor-activated receptor activity and signaling receptor binding activity. Involved in several processes, including positive regulation of intracellular signal transduction; protein phosphorylation; and vasculature development. Acts upstream of or within several processes, including animal organ development; response to ceramide; and ruffle assembly. Located in cell surface; cytoplasm; and ruffle. Is expressed in several structures, including alimentary system; extraembryonic component; genitourinary system; nervous system; and sensory organ. Used to study basal ganglia calcification; infantile myofibromatosis; myeloproliferative neoplasm; and severe nonproliferative diabetic retinopathy. Human ortholog(s) of this gene implicated in basal ganglia calcification; glioblastoma; infantile myofibromatosis; myeloproliferative neoplasm (multiple); and renal cell carcinoma. Orthologous to human PDGFRB (platelet derived growth factor receptor beta).

Rat Pdgfrb Target Information

  • Target Symbol: Pdgfrb, platelet derived growth factor receptor beta
  • Alias:
    • beta platelet-derived growth factor receptor
    • beta-type platelet-derived growth factor receptor
    • CD140 antigen-like family member B
    • PDGF-R-beta
    • PDGFR-1
    • PDGFR-beta
    • platelet derived growth factor receptor, beta polypeptide
    • platelet-derived growth factor receptor 1
    • Platelet-derived growth factor receptor beta
    • platelet-derived growth factor receptor beta variant 1
    • Platelet-derived growth factor receptor, beta
  • NCBI_Gene: 24629
  • UniProtKB: Q05030

Rat Pdgfrb Predicted Functions

Enables phosphatidylinositol 3-kinase binding activity and platelet-derived growth factor-activated receptor activity. Involved in several processes, including animal organ development; positive regulation of Rho protein signal transduction; and regulation of apoptotic process. Located in several cellular components, including apical plasma membrane; cell surface; and nucleus. Used to study extrahepatic cholestasis; middle cerebral artery infarction; and prostate cancer. Biomarker of hypertension; oligohydramnios; and sciatic neuropathy. Human ortholog(s) of this gene implicated in basal ganglia calcification; glioblastoma; infantile myofibromatosis; myeloproliferative neoplasm (multiple); and renal cell carcinoma. Orthologous to human PDGFRB (platelet derived growth factor receptor beta).

PDGFRB Target News

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