Human sP-Selectin ELISA Kit

$350.00$450.00

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TARGETSSELP
SPECIESHuman
Sample TypeSerum, plasma, cell culture supernatant, and other biological samples
Sample Volume20 μL
Sensitivity1.11 pg/mL
Range78.13 pg/mL – 5000 pg/mL
Assay Time3.5 h
Recovery88% – 106%
Average Recovery0.96
Sample TypeSerum, plasma, cell culture supernatant, and other biological samples
Sample Volume20 μL
Sensitivity1.11 pg/mL
Range78.13 pg/mL – 5000 pg/mL
Assay Time3.5 h
Recovery88% – 106%
Average Recovery0.96
Intra Precision5.3% – 7.8%
Inter-Precision5.4% – 9.0%
PlatformELISA
PlateDetachable 96-well plate
Size96T/48T
StorageIf the reagent kit is unopened, it should be stored at 4℃. However, if it has been opened, the standard solution should be stored at -20℃, while the other components should be stored at 4℃.
Delivery4℃ blue ice transportation
Components96-well polystyrene enzyme-linked immunosorbent assay (ELISA) plate coated with anti-sP-Selectin monoclonal antibody
Human sP-Selectin freeze-dried standard
sP-Selectin detect Antibody
Standard Diluent
Assay Buffer(10×)
Substrate TMB
Stop Solution
Washing Buffer(20×)
Sealing Film
Assay PrincipleThis kit utilizes the double antibody sandwich enzyme-linked immunosorbent assay (ELISA) detection technique.Specific anti-human sP-Selectin antibodies are precoated on a high-affinity ELISA plate. Standard samples, test samples, and biotinylated detection antibodies are added to the wells of the ELISA plate. After incubation, sP-Selectin present in the samples binds to the solid-phase antibodies and the detection antibodies. After washing to remove unbound substances, streptavidin-HRP labeled with horseradish peroxidase is added. After washing, a colorimetric substrate, TMB, is added and the plate is incubated in the dark for color development. The intensity of the color reaction is directly proportional to the concentration of sP-Selectin in the samples. A stop solution is added to terminate the reaction, and the absorbance value is measured at a wavelength of 450 nm (with a reference wavelength range of 570-630 nm).

 

Targets

SELP

SELP Target Infomation Overview

  • Target Symbol: SELP, selectin P
  • Gene Groups: CD molecules; Selectins; Sushi domain containing; C-type lectin domain containing
  • Alias: CD62; PSEL; PADGEM; GMP140; CD62P
  • Previous Names: GRMP
  • Alias Names: antigen CD62; granule membrane protein 140kDa; selectin P (granule membrane protein 140kD, antigen CD62); selectin P (granule membrane protein 140kDa, antigen CD62)

SELP, selectin P Target Infomation by Species

HumanMouseRat

Human SELP Target Information

  • Target Symbol: SELP, selectin P
  • Alias:
    • CD62
    • CD62 antigen-like family member P
    • CD62P
    • FLJ45155
    • GMP-140
    • GMP140
    • granule membrane protein 140
    • granule membrane protein 140kDa
    • granulocyte membrane protein
    • GRMP
    • LECAM3
    • leukocyte-endothelial cell adhesion molecule 3
    • P-selectin
    • PADGEM
    • platelet activation dependent granule-external membrane protein
    • platelet alpha-granule membrane protein
    • PSEL
    • selectin P (granule membrane protein 140kDa, antigen CD62)
  • NCBI_Gene: 6403
  • UniProtKB: P16109

Human SELP Predicted Functions

Enables several functions, including fucose binding activity; heparin binding activity; and lipopolysaccharide binding activity. Involved in several processes, including calcium-dependent cell-cell adhesion via plasma membrane cell adhesion molecules; calcium-mediated signaling using intracellular calcium source; and leukocyte tethering or rolling. Located in external side of plasma membrane; extracellular space; and platelet alpha granule membrane. Is integral component of plasma membrane. Implicated in atopic dermatitis and myocardial infarction. Biomarker of several diseases, including Kawasaki disease; autoimmune disease (multiple); coronary artery disease (multiple); obesity; and type 2 diabetes mellitus.

Mouse Selp Target Information

Mouse Selp Predicted Functions

Enables calcium-dependent protein binding activity and sialic acid binding activity. Involved in positive regulation of leukocyte tethering or rolling and positive regulation of platelet activation. Acts upstream of or within several processes, including heterophilic cell-cell adhesion via plasma membrane cell adhesion molecules; leukocyte tethering or rolling; and positive regulation of leukocyte migration. Located in external side of plasma membrane. Is expressed in blood and liver. Human ortholog(s) of this gene implicated in atopic dermatitis and myocardial infarction. Orthologous to human SELP (selectin P).

Rat Selp Target Information

Rat Selp Predicted Functions

Predicted to enable several functions, including fucose binding activity; heparin binding activity; and lipopolysaccharide binding activity. Involved in several processes, including leukocyte migration; regulation of cellular extravasation; and response to lipopolysaccharide. Located in external side of plasma membrane and extracellular space. Used to study acute necrotizing pancreatitis and anti-basement membrane glomerulonephritis. Biomarker of atherosclerosis; colitis; congestive heart failure; and myocardial infarction. Human ortholog(s) of this gene implicated in atopic dermatitis and myocardial infarction. Orthologous to human SELP (selectin P).

SELP Target News

    Cardiovascular Disease (Hypertension & Atherosclerosis & Acute Myocardial Infarction & Coronary Heart Disease)
    Obesity Diabetes
    Osteoarthrosis & Rheumatoid Arthritis
    Sepsis
    Systemic Lupus Erythematosus

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