Mouse Adiponectin/Acrp30 ELISA Kit

$350.00$450.00

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TARGETSADIPOQ
SPECIESMouse
Sample TypeSerum, plasma, cell culture supernatant, and other biological samples
Sample Volume10 μL
Sensitivity0.73 pg/mL
Range31.25 pg/mL – 2000 pg/mL
Assay Time3.5 h
Recovery92% – 113%
Average Recovery1.07
Sample TypeSerum, plasma, cell culture supernatant, and other biological samples
Sample Volume10 μL
Sensitivity0.73 pg/mL
Range31.25 pg/mL – 2000 pg/mL
Assay Time3.5 h
Recovery92% – 113%
Average Recovery1.07
Intra Precision5.1% – 8.8%
Inter-Precision7.3% – 7.7%
PlatformELISA
PlateDetachable 96-well plate
Size96T/48T
StorageIf the reagent kit is unopened, it should be stored at 4℃. However, if it has been opened, the standard solution should be stored at -20℃, while the other components should be stored at 4℃.
Delivery4℃ blue ice transportation
Components96-well polystyrene enzyme-linked immunosorbent assay (ELISA) plate coated with anti-Adiponectin/Acrp30 monoclonal antibody
Mouse Adiponectin/Acrp30 freeze-dried standard
Adiponectin/Acrp30 detect Antibody
Standard Diluent
Assay Buffer(10×)
Substrate TMB
Stop Solution
Washing Buffer(20×)
Sealing Film
Assay PrincipleThis kit utilizes the double antibody sandwich enzyme-linked immunosorbent assay (ELISA) detection technique.Specific anti-mouse Adiponectin antibodies are precoated on a high-affinity ELISA plate. Standard samples, test samples, and biotinylated detection antibodies are added to the wells of the ELISA plate. After incubation, Adiponectin present in the samples binds to the solid-phase antibodies and the detection antibodies. After washing to remove unbound substances, streptavidin-HRP labeled with horseradish peroxidase is added. After washing, a colorimetric substrate, TMB, is added and the plate is incubated in the dark for color development. The intensity of the color reaction is directly proportional to the concentration of Adiponectin in the samples. A stop solution is added to terminate the reaction, and the absorbance value is measured at a wavelength of 450 nm (with a reference wavelength range of 570-630 nm).

 

Targets

ADIPOQ

ADIPOQ Target Infomation Overview

  • Target Symbol: ADIPOQ, adiponectin, C1Q and collagen domain containing
  • Gene Groups: C1q and TNF related
  • Alias: ACRP30; apM1; GBP28; adiponectin
  • Previous Names: ACDC
  • Alias Names: adipose most abundant gene transcript 1; adiponectin precursor; adipocyte, C1Q and collagen domain containing

ADIPOQ, adiponectin, C1Q and collagen domain containing Target Infomation by Species

HumanMouseRat

Human ADIPOQ Target Information

  • Target Symbol: ADIPOQ, adiponectin, C1Q and collagen domain containing
  • Alias:
    • 30 kDa adipocyte complement-related protein
    • ACDC
    • ACRP30
    • adipocyte complement-related 30 kDa protein
    • adiponectin
    • adipose most abundant gene transcript 1 protein
    • adipose specific collagen-like factor
    • ADIPQTL1
    • ADPN
    • APM-1
    • APM1
    • GBP28
    • gelatin-binding protein 28
  • NCBI_Gene: 9370
  • UniProtKB: Q15848

Human ADIPOQ Predicted Functions

Enables hormone activity; protein homodimerization activity; and sialic acid binding activity. Involved in several processes, including negative regulation of cell communication; negative regulation of cell differentiation; and negative regulation of macromolecule metabolic process. Located in cell surface and extracellular space. Part of collagen-containing extracellular matrix. Implicated in several diseases, including carcinoma (multiple); cardiovascular system disease (multiple); non-alcoholic fatty liver disease; primary open angle glaucoma; and type 2 diabetes mellitus. Biomarker of several diseases, including autoimmune disease (multiple); cardiovascular system disease (multiple); diabetes mellitus (multiple); fatty liver disease (multiple); and inflammatory bowel disease (multiple).

Mouse Adipoq Target Information

Mouse Adipoq Predicted Functions

Enables hormone activity and identical protein binding activity. Involved in several processes, including detection of oxidative stress; negative regulation of cold-induced thermogenesis; and regulation of protein serine/threonine kinase activity. Acts upstream of or within with a positive effect on gene expression. Acts upstream of or within several processes, including brown fat cell differentiation; negative regulation of gluconeogenesis; and positive regulation of glucose import. Located in endoplasmic reticulum and extracellular space. Is expressed in several structures, including adipose tissue; axial musculature; gut; male reproductive gland or organ; and serum. Human ortholog(s) of this gene implicated in several diseases, including carcinoma (multiple); cardiovascular system disease (multiple); non-alcoholic fatty liver disease; primary open angle glaucoma; and type 2 diabetes mellitus. Orthologous to human ADIPOQ (adiponectin, C1Q and collagen domain containing).

Rat Adipoq Target Information

Rat Adipoq Predicted Functions

Enables identical protein binding activity. Involved in several processes, including cellular response to organonitrogen compound; positive regulation of glycogen (starch) synthase activity; and positive regulation of protein phosphorylation. Located in extracellular space. Part of protein-containing complex. Used to study brain infarction. Biomarker of several diseases, including end stage renal disease; fatty liver disease (multiple); glucose metabolism disease (multiple); hyperthyroidism; and obesity. Human ortholog(s) of this gene implicated in several diseases, including carcinoma (multiple); cardiovascular system disease (multiple); non-alcoholic fatty liver disease; primary open angle glaucoma; and type 2 diabetes mellitus. Orthologous to human ADIPOQ (adiponectin, C1Q and collagen domain containing).

ADIPOQ Target News

    5.834
    CircHOMER1 inhibits porcine adipogenesis via the miR-23b/SIRT1 axis
    FASEB JOURNAL
    6.6
    Injectable cell-targeting fiber rods to promote lipolysis and inflammatory regulation for obesity treatment
    Biomaterials Science
    7.9
    SIRT1 inhibitors within Qing-Luo-Yin alleviated white adipose tissues-mediated inflammation in antigen-induced arthritis mice
    PHYTOMEDICINE
    Tumor Marker

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