EasyGo!™ Mouse IL-6 One-Step ELISA Kit

$400.00$500.00

TARGETS IL6
SPECIES Mouse
Sample Type Serum, plasma, cell culture supernatant
Sample Volume Serum or plasma: 10μL;  cell culture supernatant: 50μL
Sensitivity 4.92 pg/mL
Range 7.81 pg/mL – 500 pg/mL
Assay Time 1.5 h
Recovery 89% – 112%
Average Recovery 1.02
Sample Type Cell culture supernates, serum and plasma
Sample Volume Serum or plasma: 10μL;  cell culture supernatant: 50μL
Sensitivity 4.92 pg/mL
Range 7.81 pg/mL – 500 pg/mL
Assay Time 1.5 h
Recovery 89% – 112%
Average Recovery 1.02
Intra Precision 2.3% – 3.0%
Inter-Precision 2.1% – 5.3%
Platform ELISA
Plate Detachable 96-well plate
Size 96T/48T
Storage If the reagent kit is unopened, it should be stored at 4℃. However, if it has been opened, the standard solution should be stored at -20℃, while the other components should be stored at 4℃.
Delivery 4℃ blue ice transportation
Components 96-well polystyrene enzyme-linked immunosorbent assay (ELISA) plate coated with anti-IL-6 monoclonal antibody
Mouse IL-6 freeze-dried standard
IL-6 detect Antibody
Standard Diluent
Assay Buffer(10×)
Substrate TMB
Stop Solution
Washing Buffer(20×)
Sealing Film
Assay Principle This assay employs the quantitative sandwich enzyme immunoassay technique. Amonoclonal antibody specific for Mouse IL-6 has been immobilized onto microwells, and two pellets of the biotin-linked detect antibody specific for IL-6 (light yellow) and streptavidin-HRP (purple) are pre-placed in the microwells, sealed by the adhesive film. Standard or samples are pipetted intothe wells, then IL-6 present is bound by the immobilized antibody and detect antibody, of whichthe latter is conjugated with streptavidin-HRP in the incubation. After washing, substrate solutionreacts with HRP and color develops in proportion to the amount of IL-6 bound by the immobilizedantibody. The color development is stopped and the intensity of the color is measured bymicroplate reader.

Targets

IL6

IL6 Target Infomation Overview

  • Target Symbol: IL6, interleukin 6
  • Gene Groups: Interferons; Interleukins; Interleukin 6 type cytokine family
  • Alias: IL-6; BSF2; HGF; HSF
  • Previous Names: IFNB2
  • Alias Names: interferon, beta 2; interleukin 6 (interferon, beta 2)

IL6, interleukin 6 Target Infomation by Species

[su_tabs][su_tab title=”Human” disabled=”no” anchor=”” url=”” target=”blank” class=”tab-human”]

Human IL6 Target Information

  • Target Symbol: IL6, interleukin 6
  • Alias:
    • B cell stimulatory factor-2
    • B-cell differentiation factor
    • B-cell stimulatory factor 2
    • BSF-2
    • BSF2
    • CDF
    • CTL differentiation factor
    • HGF
    • HSF
    • hybridoma growth factor
    • IFN-beta-2
    • IFNB2
    • IL-6
    • interferon beta-2
    • interferon, beta 2
    • interleukin BSF-2
    • interleukin-6
  • NCBI_Gene: 3569
  • UniProtKB: P05231

Human IL6 Predicted Functions

Enables cytokine activity and growth factor activity. Contributes to interleukin-6 receptor binding activity. Involved in several processes, including positive regulation of cell population proliferation; positive regulation of macromolecule metabolic process; and regulation of peptide hormone secretion. Acts upstream of or within several processes, including cellular response to lipopolysaccharide; positive regulation of leukocyte adhesion to vascular endothelial cell; and positive regulation of macromolecule metabolic process. Located in extracellular space. Part of interleukin-6 receptor complex. Implicated in several diseases, including autoimmune disease (multiple); eye disease (multiple); gastrointestinal system cancer (multiple); glucose metabolism disease (multiple); and periodontal disease (multiple). Biomarker of several diseases, including autoimmune disease (multiple); eye disease (multiple); gastrointestinal system cancer (multiple); glucose metabolism disease (multiple); and lung disease (multiple).

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[su_tab title=”Mouse” disabled=”no” anchor=”” url=”” target=”blank” class=”tab-mouse”]

Mouse Il6 Target Information

Mouse Il6 Predicted Functions

Enables cytokine activity and interleukin-6 receptor binding activity. Involved in several processes, including animal organ development; positive regulation of macromolecule metabolic process; and regulation of peptide hormone secretion. Acts upstream of or within with a positive effect on gene expression. Acts upstream of or within several processes, including cellular response to cytokine stimulus; regulation of gene expression; and salivary gland morphogenesis. Located in external side of plasma membrane and extracellular space. Part of interleukin-6 receptor complex. Is expressed in several structures, including adrenal gland; genitourinary system; gut; liver; and thymus. Human ortholog(s) of this gene implicated in several diseases, including artery disease (multiple); autoimmune disease (multiple); gastrointestinal system cancer (multiple); glucose metabolism disease (multiple); and periodontal disease (multiple). Orthologous to human IL6 (interleukin 6).

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[su_tab title=”Rat” disabled=”no” anchor=”” url=”” target=”blank” class=”tab-rat”]

Rat Il6 Target Information

  • Target Symbol: Il6, interleukin 6
  • Alias:
    • Ifnb2
    • IL-6
    • ILg6
    • Interleukin 6 (interferon beta 2)
    • Interleukin 6 (interferon, beta 2)
    • interleukin-6
  • NCBI_Gene: 24498
  • UniProtKB: P20607

Rat Il6 Predicted Functions

Enables cytokine activity and interleukin-6 receptor binding activity. Involved in several processes, including cellular response to lipid; positive regulation of cell communication; and response to peptide hormone. Located in cytoplasm and extracellular space. Used to study several diseases, including acute necrotizing pancreatitis; disseminated intravascular coagulation; fatty liver disease; hypertension (multiple); and impotence. Biomarker of several diseases, including acute necrotizing pancreatitis; artery disease (multiple); auditory system disease (multiple); iron deficiency anemia; and lung disease (multiple). Human ortholog(s) of this gene implicated in several diseases, including autoimmune disease (multiple); eye disease (multiple); gastrointestinal system cancer (multiple); glucose metabolism disease (multiple); and periodontal disease (multiple). Orthologous to human IL6 (interleukin 6).

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IL6 Target News

[catlist tags=”il6″ template=targetnews thumbnail=yes thumbnail_class=”related-post-media clr” thumbnail_size=833]

6.9
Controlling the speed of antigens transport in dendritic cells improves humoral and cellular immunity for vaccine
BIOMEDICINE & PHARMACOTHERAPY
5.7
SPI1-mediated macrophage polarization aggravates age-related macular degeneration
Frontiers in Immunology
13.3
Alginate/silk fibroin/Zn2+ composite microspheres for site-specific delivery for enhanced ulcerative colitis therapy
CHEMICAL ENGINEERING JOURNAL
2.9
Exploration of Antimicrobial Peptides in the Treatment of Gentamicin-Resistant Klebsiella pneumoniae Infection
Infection and Drug Resistance
2.9
Near-infrared-II responsive ovalbumin functionalized gold-genipin nanosystem cascading photo-immunotherapy of cancer
NANOTECHNOLOGY
5.8
Interfacial engineered living drugs with an “ON/OFF” switching for oral delivery
Nanoscale
5.5
Biomimetic NO Scavenging Hyaluronic Acid Nanoparticles Enable Targeted Delivery of MTX and Integrated Management of Rheumatoid Arthritis
BIOMACROMOLECULES
7.6
Mediation of FOXA2/IL-6/IL-6R/STAT3 signaling pathway mediates benzo[a]pyrene-induced airway epithelial mesenchymal transformation in asthma
ENVIRONMENTAL POLLUTION
6.2
Selenium deficiency induces irritable bowel syndrome: Analysis of UK Biobank data and experimental studies in mice
ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
12.8
Engineered low-pathogenic Helicobacter pylori as orally tumor immunomodulators for the stimulation of systemic immune response
BIOMATERIALS
14.3
Biomimetic Hydrogel-Mediated Mechano-Immunometabolic Therapy for Inhibition of ccRCC Recurrence After Surgery
Advanced Science
3.8
Oligomeric proanthocyanidins mitigate acute lung injury by inhibiting NETs and inflammation via the gut-lung axis
Journal of Functional Foods
7.7
Structural characterization and adjuvant activity of a water soluble polysaccharide from Poria cocos
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
5.3
Chloranthalactone B covalently binds to the NACHT domain of NLRP3 to attenuate NLRP3-driven inflammation
BIOCHEMICAL PHARMACOLOGY
6.9
ABBV-744 alleviates LPS-induced neuroinflammation via regulation of BATF2-IRF4-STAT1/3/5 axis
ACTA PHARMACOLOGICA SINICA
8.5
The influences of extraction methods on the chemical constituents of Lyonia ovalifolia (wall.) Drude and intracellular protective effects based on metabolomics analysis
FOOD CHEMISTRY
Allergic Reaction
Cardiovascular Disease (Hypertension & Atherosclerosis & Acute Myocardial Infarction & Coronary Heart Disease)
Eye Diseases (Glaucoma & Cataract)
Lung Disease (Asthma & COPD)
Multiple Sclerosis
Nervous System Diseases (Depression & Alzheimer & Epilepsy & Parkinson)
Obesity Diabetes
Osteoarthrosis & Rheumatoid Arthritis
Osteoporosis
Sepsis
Systemic Lupus Erythematosus
Transplantation
Tumor Marker

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