MULTI SCIENCES
96 Wells Mouse IL-4 ELISA Kit
96 Wells Mouse IL-4 ELISA Kit
SKU:EK204
Product Details
| Brand | MultiSciences |
|---|---|
| CatNum | 70-EK204 |
| Product Name | Mouse IL-4 ELISA Kit |
| Customs Name | Mouse IL-4 ELISA Kit |
| Application | ELISA |
| Reactivity | Mouse |
| Assay Type | Sandwich ELISA |
| Suitable Sample Type | serum, plasma, cell culture supernates |
| Format | 96-well strip plate |
| Storage | 4℃ (unopened) standard stored at -20℃, others stored at 4℃ (opened) |
| Shipping Condition | 4℃ |
| Sample Volume | 20 μl |
| Sensitivity | 0.34 pg/ml |
| Standard Curve Range | 7.81 - 500 pg/ml |
| Spike Recovery Range | 93 % - 106 % |
| Mean Spike Recovery | 100% |
| CV of Intra plate | 6.8 % - 8.3 % |
| CV of Inter plate | 3.5 % - 6.6 % |
| Components | 96-well polystyrene microplate coated with a monoclonal antibody against Mouse IL-4 Mouse IL-4 Standard, lyophilized Mouse IL-4 Detect Antibody Standard Diluent Streptavidin-HRP Assay Buffer (10×) Substrate (TMB) Stop Solution washing Buffer (20×) Plate Covers |
| Describtion | This assay employs the quantitative sandwich enzyme immunoassay technique for the quantitative detection of mouse IL-4. The Mouse IL-4 ELISA is for research use only. Not for diagnostic or therapeutic procedures. Interleukin 4 (IL-4) is an approximately 18-20 kDa monomeric cytokine that induces differentiation of naive helper T cells (Th0 cells) to Th2 cells. Mouse IL-4 is synthesized as a 140 amino acid (aa) precursor with a 20 aa signal sequence and a 120 aa mature chain and mature mouse IL-4 shares 43%, and 63% aa sequence identity with human and rat IL-4. IL-4 is a cytokine that induces differentiation of naive helper T cells (Th0 cells) to Th2 cells. Upon activation by IL-4, Th2 cells subsequently produce additional IL-4 in a positive feedback loop. The cell that initially produces IL-4, thus inducing Th0 differentiation, has not been identified, but recent studies suggest that basophils may be the effector cell. It is closely related and has functions similar to IL-13. IL-4 also has been shown to drive mitogenesis, dedifferentiation, and metastasis in rhabdomyosarcoma. IL-4, along with other Th2 cytokines, is involved in the airway inflammation observed in the lungs of patients with allergic asthma. |
