MULTI SCIENCES
Human ACE ELISA Kit
Human ACE ELISA Kit
SKU:EK1317
Product Details
| Factory Name | MultiSciences |
|---|---|
| Product Code | 70-EK1317-96 |
| CatNum | 70-EK1317-96 |
| Product Name | Human ACE ELISA Kit |
| Customs Name | Human ACE ELISA Kit |
| Product Spec | 96T |
| SalePrice | 3200 |
| Type | ELISA Kit |
| Application | ELISA |
| Reactivity | Human |
| Modality | NoData |
| Assay Type | Sandwich ELISA |
| Suitable Sample Type | serum, plasma, cell culture supernates |
| Format | 96-well strip plate |
| Storage | 4℃ (unopened) standard stored at -20℃, others stored at 4℃ (opened) |
| Shipping Condition | 4℃ |
| Sensitivity | 8.34 pg/ml |
| Standard Curve Range | 125 - 8000 pg/ml |
| Spike Recovery Range | 90 %-110 % |
| Mean Spike Recovery | 103% |
| CV of Intra plate | 2.6 % - 3.5 % |
| CV of Inter plate | 2.7 % - 4.2 % |
| Components |
96-well polystyrene microplate (12 strips of 8 wells) coated with an antibody against human ACE Human ACE Standard, lyophilized ACE Detect Antibody Streptavidin-HRP Assay Buffer (10×) Substrate Stop Solution Washing Buffer (20×) Adhesive Films |
| PRINCIPLE OF THE ASSAY | Human ACE ELISA Kit is based on the quantitative sandwich enzyme-linked immunosorbent assay technique to measure concentration of human ACE in the samples. An antibody specific for human ACE has been immobilized onto microwells. Standard or samples are pipetted into the wells, followed by the addition of biotin-linked detect antibody specific for ACE, and ACE present is bound by the immobilized antibody and detect antibody following the first incubation. After removal of any unbound substances, streptavidin-HRP is added for a second incubation. After washing, substrate solution reacts with HRP and color develops in proportion to the amount of ACE bound by the immobilized antibody. The color development is stopped by addition of acid and the optical density value is measured by microplate reader. |
| Describtion |
Angiotensin I Converting Enzyme (ACE), also known as peptidyl-dipetidase A, is a zinc metallopeptidase important for blood pressure control and water and salt metabolism. ACE converts angiotensin I to angiotensin II by release of the terminal His-Leu dipeptide, this results in an increase of the vasoconstrictor activity of angiotensin. It is also able to inactivate bradykinin, a potent vasodilator. ACE and ACE-2, two cell surface proteases, are important regulators of the renin-angiotensin system (RAS), which plays a key role in maintaining blood pressure homeostasis and fluid salt balance in mammals. ACE exists in two isoforms. Somatic ACE, found in endothelial, epithelial and neuronal cells, comprises two highly similar catalytic domains called N- and C-domains whereas germinal ACE comprises a single protease domain. ACE-2 consists of a single protease domain and is an essential regulator of heart and lung function. It is also a cellular receptor for the Spike protein of SARS cornonavirus that causes severe acute respiratory syndrome. |
