MULTI SCIENCES
Human Adiponectin ELISA Kit Distributor
Human Adiponectin ELISA Kit Distributor
SKU:EK195
Product Details
| Brand | MultiSciences |
|---|---|
| CatNum | 70-EK195 |
| Product Name | Human Adiponectin ELISA Kit |
| Customs Name | Human Adiponectin ELISA Kit |
| Application | ELISA |
| Reactivity | Human |
| Assay Type | Sandwich ELISA |
| Suitable Sample Type | serum, plasma, cell culture supernates |
| Format | 96-well strip plate |
| Storage | 4℃ (unopened) standard stored at -20℃, others stored at 4℃ (opened) |
| Shipping Condition | 4℃ |
| Sample Volume | 50 μl (prediluted) |
| Sensitivity | 0.44 pg/ml |
| Standard Curve Range | 62.50 - 4000 pg/ml |
| Spike Recovery Range | 95 % - 106 % |
| Mean Spike Recovery | 0.99 |
| CV of Intra plate | 3.4 % - 4.9 % |
| CV of Inter plate | 2.4 % - 5.7 % |
| Components | 96-well polystyrene microplate coated with a monoclonal antibody against Adiponectin Human Adiponectin Standard, lyophilized Adiponectin Detect Antibody Streptavidin-HRP Assay Buffer (10×) Substrate (TMB) Stop Solution washing Buffer (20×) Plate Covers |
| Describtion | This assay employs the quantitative sandwich enzyme immunoassay technique for the quantitative detection of human Adiponectin. The Human Adiponectin ELISA is for research use only. Not for diagnostic or therapeutic procedures. Adiponectin, also known as gelatin-binding protein of 28 kDa (GBP-28), adipose most abundant gene transcript 1 (apM1), Adipocyte complement-related protein of 30 kDa (Acrp30), and AdipoQ, is a protein involved in regulating glucose levels as well as fatty acid breakdown. There are four distinct regions of adiponectin: a short signal sequence that targets the hormone for secretion outside the cell, a short region that varies between species, a 65-amino acid region with similarity to collagenous proteins and a globular domain. Adiponectin assembles into different complexes including trimers, hexamers, and higher order oligomeric structures that may affect biological activity. Adiponectin is a protein hormone that modulates a number of metabolic processes, including glucose regulation and fatty acid oxidation and is exclusively secreted from adipose tissue (and also from the placenta in pregnancy) into the bloodstream and is very abundant in plasma relative to many hormones. Adiponectin may also play anti-atherogenic and anti-inflammatory roles, and negatively regulates myelomonocytic progenitor cell growth and TNF-α production in macrophages. Monitoring of Adiponectin levels in serum provides more detailed insights in several pathological situations such as type 2 diabetes, obesity, atherosclerosis and non-alcoholic fatty liver disease (NAFLD). |
