MultiSciences

70-EK1204

Human Apolipoprotein A1/Apo A1 ELISA Kit

Human Apolipoprotein A1/Apo A1 ELISA Kit

ELISA

Human

Sandwich ELISA

serum, plasma, cell culture supernates

96-well strip plate

4℃ (unopened)standard stored at -20℃, others stored at 4℃ (opened)

4℃

10 μl (prediluted)

0.73 ng/ml

3.13 - 200 ng/ml

-

-

3.4 % - 6.9 %

2.0 % - 6.6 %

96-well polystyrene microplate coated with a monoclonal antibody against Apo A1
Human Apo A1 Standard, lyophilized
Apo A1 Detect Antibody
Streptavidin-HRP
Assay Buffer (10×)
Substrate (TMB)
Stop Solution
Washing Buffer (20×)
Plate Covers

This assay employs the quantitative sandwich enzyme immunoassay technique for the quantitative detection of human Apo A1. The Human Apolipoprotein A1/Apo A1 ELISA is for research use only. Not for diagnostic or therapeutic procedures.

Apolipoprotein A1 (Apo A1) is the major protein component of high density lipoprotein (HDL) in plasma. Chylomicrons secreted from the intestinal enterocyte also contain Apo A1, but it is quickly transferred to HDL in the bloodstream. The protein has a specific role in lipid metabolism. It promotes fat efflux, including cholesterol, from tissues to the liver for excretion. It is a cofactor for lecithin cholesterolacyltransferase (LCAT) which is responsible for the formation of most plasma cholesteryl esters. Apo A1 was also isolated as a prostacyclin (PGI2) stabilizing factor, and thus may have an anticlotting effect.

Apo A1 is often used as a biomarker for prediction of cardiovascular diseases and the ratio Apo B-100/Apo A1 has been reported as a stronger predictor for the risk of myocardial infarction than any other lipid measurement. Defects in the gene encoding it are associated with HDL deficiencies, including Tangier disease, and with systemic non-neuropathic amyloidosis.