|Product Name||Human Apolipoprotein D/Apo D ELISA Kit|
|Customs Name||Human Apolipoprotein D/Apo D ELISA Kit|
|Assay Type||Sandwich ELISA|
|Suitable Sample Type||serum, plasma, cell culture supernates|
|Format||96-well strip plate|
|Storage||4℃ (unopened) standard stored at -20℃, others stored at 4℃ (opened)|
|Sample Volume||10 μl (prediluted)|
|Standard Curve Range||625 - 40000 pg/ml|
|Spike Recovery Range||-|
|Mean Spike Recovery||-|
|CV of Intra plate||2.0 % - 8.1 %|
|CV of Inter plate||1.9 % - 2.8 %|
|Components||96-well polystyrene microplate coated with a monoclonal antibody against Apo D
Human Apo D Standard, lyophilized
Apo D Detect Antibody
Assay Buffer (10×)
Washing Buffer (20×)
|Describtion||This assay employs the quantitative sandwich enzyme immunoassay technique for the quantitative detection of human Apo D. The Human Apolipoprotein D/Apo D ELISA is for research use only. Not for diagnostic or therapeutic procedures.
Apolipoprotein D (Apo D) is a glycoprotein of estimated molecular weight 33 kDa that in humans is encoded by the APOD gene. Unlike other lipoproteins, which are mainly produced in the liver, Apo D is mainly produced in the brain and testes. It is a component of high-density lipoprotein (HDL) that has no marked similarity to other apolipoprotein sequences. Apo D is closely associated with the enzyme lecithin:cholesterol acyltransferase - an enzyme involved in lipoprotein metabolism. Apo D has also been shown to be an important link in the transient interaction between HDL and LDL particles and between HDL particles and cells.
APO D is a biomarker of androgen insensitivity syndrome (AIS). It is associated with neurological disorders and nerve injury, especially related to myelin sheath. APO D was shown to be elevated in a rat model of stroke. APO D is elevated in patients with schizophrenia, bipolar disorder, and Alzheimer's disease.