MULTI SCIENCES
Human Apolipoprotein E/Apo Cytokine E ELISA Kit
Human Apolipoprotein E/Apo Cytokine E ELISA Kit
SKU:EK1218
Product Details
| Brand | MultiSciences |
|---|---|
| CatNum | 70-EK1218 |
| Product Name | Human Apolipoprotein E/Apo E ELISA Kit |
| Customs Name | Human Apolipoprotein E/Apo E ELISA Kit |
| Application | ELISA |
| Reactivity | Human |
| Assay Type | Sandwich ELISA |
| Suitable Sample Type | serum, plasma, cell culture supernates |
| Format | 96-well strip plate |
| Storage | 4℃ (unopened) standard stored at -20℃, others stored at 4℃ (opened) |
| Shipping Condition | 4℃ |
| Sample Volume | 10 μl (prediluted) |
| Sensitivity | 1.71 pg/ml |
| Standard Curve Range | 78.13 - 5000 pg/ml |
| Spike Recovery Range | - |
| Mean Spike Recovery | - |
| CV of Intra plate | 3.5 % - 5.0 % |
| CV of Inter plate | 3.8 % - 5.0 % |
| Components | 96-well polystyrene microplate coated with a monoclonal antibody against Apo E Human Apo E Standard, lyophilized Apo E Detect Antibody Streptavidin-HRP Assay Buffer (10×) Substrate (TMB) Stop Solution Washing Buffer (20×) Plate Covers |
| Describtion | This assay employs the quantitative sandwich enzyme immunoassay technique for the quantitative detection of human Apo E. The Human Apolipoprotein E / Apo E ELISA is for research use only. Not for diagnostic or therapeutic procedures. Apolipoprotein E (Apo E) is a class of apolipoprotein found in the chylomicron and Intermediate-density lipoprotein that is essential for the normal catabolism of triglyceride-rich lipoprotein constituents. In peripheral tissues, Apo E is primarily produced by the liver and macrophages, and mediates cholesterol metabolism in an isoform-dependent manner. In the central nervous system, Apo E is mainly produced by astrocytes, and transports cholesterol to neurons via Apo E receptors. Apo E transports lipoproteins, fat-soluble vitamins, and cholesterol into the lymph system and then into the blood. In the field of immune regulation, a growing number of studies point to Apo E's interaction with many immunological processes, including suppressing T cell proliferation, macrophage functioning regulation, lipid antigen presentation facilitation to natural killer T cell as well as modulation of inflammation and oxidation. The secretion of Apo E by monocytes is down regulated by inflammatory cytokines and upregulated by TGF-β. This protein is involved in Alzheimer's disease and cardiovascular disease. |
