MULTI SCIENCES
Human Apolipoprotein J/Apo J ELISA Kit plate
Human Apolipoprotein J/Apo J ELISA Kit plate
SKU:EK1221
Product Details
| Brand | MultiSciences |
|---|---|
| CatNum | 70-EK1221 |
| Product Name | Human Apolipoprotein J/Apo J ELISA Kit |
| Customs Name | Human Apolipoprotein J/Apo J ELISA Kit |
| Application | ELISA |
| Reactivity | Human |
| Assay Type | Sandwich ELISA |
| Suitable Sample Type | serum, plasma, cell culture supernates |
| Format | 96-well strip plate |
| Storage | 4℃ (unopened) standard stored at -20℃, others stored at 4℃ (opened) |
| Shipping Condition | 4℃ |
| Sample Volume | 100 μl (prediluted) |
| Sensitivity | 2.08 ng/ml |
| Standard Curve Range | 31.25 - 2000 ng/ml |
| Spike Recovery Range | - |
| Mean Spike Recovery | - |
| CV of Intra plate | 4.6 % - 4.9 % |
| CV of Inter plate | 2.4 % - 5.1 % |
| Components | 96-well polystyrene microplate coated with a monoclonal antibody against Apo J Human Apo J Standard, lyophilized Apo J Detect Antibody Streptavidin-HRP Assay Buffer (10×) Substrate (TMB) Stop Solution Washing Buffer (20×) Plate Covers |
| Describtion | This assay employs the quantitative sandwich enzyme immunoassay technique for the quantitative detection of human Apo J. The Human Apolipoprotein J / Apo J ELISA is for research use only. Not for diagnostic or therapeutic procedures. Apolipoprotein J (Apo J), also known as clusterin, is a 75 - 80 kDa disulfide-linked heterodimeric protein. It is a molecular chaperone responsible for aiding protein folding of secreted proteins, and its three isoforms have been differentially implicated in pro- or antiapoptotic processes. Overexpression of the secretory Apo J isoform protects the cell from apoptosis induced by cellular stress, such as chemotherapy, radiotherapy, or androgen/estrogen depletion. Apo J promotes cell survival by a number of means, including inhibition of BAX on the mitochondrial membrane, activation of the phosphatidylinositol 3-kinase/protein kinase B pathway, promotion of angiogenesis, and mediation of the nuclear factor kappa B (NF-κB) pathway. Apo J is involved in many diseases related to oxidative stress, including neurodegenerative diseases, cancers, inflammatory diseases, and aging. Studies have suggested that people who already have Alzheimer's disease have more Apo J in their blood, and Apo J levels in blood correlate with faster cognitive decline in individuals with Alzheimer's disease. |
