|Product Name||Human Apolipoprotein J/Apo J ELISA Kit|
|Customs Name||Human Apolipoprotein J/Apo J ELISA Kit|
|Assay Type||Sandwich ELISA|
|Suitable Sample Type||serum, plasma, cell culture supernates|
|Format||96-well strip plate|
|Storage||4℃ (unopened) standard stored at -20℃, others stored at 4℃ (opened)|
|Sample Volume||100 μl (prediluted)|
|Standard Curve Range||31.25 - 2000 ng/ml|
|Spike Recovery Range||-|
|Mean Spike Recovery||-|
|CV of Intra plate||4.6 % - 4.9 %|
|CV of Inter plate||2.4 % - 5.1 %|
|Components||96-well polystyrene microplate coated with a monoclonal antibody against Apo J
Human Apo J Standard, lyophilized
Apo J Detect Antibody
Assay Buffer (10×)
Washing Buffer (20×)
|Describtion||This assay employs the quantitative sandwich enzyme immunoassay technique for the quantitative detection of human Apo J. The Human Apolipoprotein J / Apo J ELISA is for research use only. Not for diagnostic or therapeutic procedures.
Apolipoprotein J (Apo J), also known as clusterin, is a 75 - 80 kDa disulfide-linked heterodimeric protein. It is a molecular chaperone responsible for aiding protein folding of secreted proteins, and its three isoforms have been differentially implicated in pro- or antiapoptotic processes. Overexpression of the secretory Apo J isoform protects the cell from apoptosis induced by cellular stress, such as chemotherapy, radiotherapy, or androgen/estrogen depletion. Apo J promotes cell survival by a number of means, including inhibition of BAX on the mitochondrial membrane, activation of the phosphatidylinositol 3-kinase/protein kinase B pathway, promotion of angiogenesis, and mediation of the nuclear factor kappa B (NF-κB) pathway.
Apo J is involved in many diseases related to oxidative stress, including neurodegenerative diseases, cancers, inflammatory diseases, and aging. Studies have suggested that people who already have Alzheimer's disease have more Apo J in their blood, and Apo J levels in blood correlate with faster cognitive decline in individuals with Alzheimer's disease.