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Human Artemin ELISA Kit

Human Artemin ELISA Kit


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Product Details

Human Artemin ELISA Kit

Factory Name MultiSciences
Product Code 70-EK1281 - 96
Cat Num EK1281 - 96
Product Name Human Artemin ELISA Kit
Customs Name Human Artemin ELISA Kit
Product Spec 96 T
Type ELISA Kit
Application ELISA
Reactivity Human
SDS SDS - EK1281
Assay Type Sandwich ELISA
Suitable Sample Type serum, plasma, cell culture supernates
Format 96-well strip plate
Storage 4℃ (unopened)standard stored at -20℃, others stored at 4℃ (opened)
Shipping Condition 4℃
Sample Volume 100ul
Sensitivity 2.63 pg/ml 
Standard Curve Range 125 - 8000 pg/ml
Spike Recovery Range 85 % - 127 % 
Mean Spike Recovery 97%
CV of Intra plate 3.5% - 4.5%
CV of Inter plate 3.3% - 4.7%

96-well polystyrene microplate coated with a monoclonal antibody against Artemin

Human Artemin Standard, lyophilized

Artemin Detect Antibody

Standard Diluent

Assay Buffer (10×)

Substrate (TMB)

Stop Solution

Washing Buffer (20×)

Plate Covers

Describtion This assay employs the quantitative sandwich enzyme immunoassay technique. A monoclonal antibody specific for human Artemin has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and Artemin present is bound by the immobilized antibody. After washing away any unbound substances, a biotin-linked detect antibody specific for Artemin is added to the wells. Following a wash to remove any unbound antibody-biotin reagent, streptavidin-HRP is added. After washing, substrate solution is added to the wells and color develops in proportion to the amount of Artemin bound in the initial step. The color development is stopped and the intensity of the color is measured.