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Human B7-1/CD80 ELISA Kit

Human B7-1/CD80 ELISA Kit


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Product Details

Human B7-1/CD80 ELISA Kit

Factory Name MultiSciences
Product Code 70-EK1272-96
CatNum EK1272-96
Product Name Human B7-1/CD80 ELISA Kit
Customs Name Human B7-1/CD80 ELISA Kit
Product Spec 96 T
Type ELISA Kit
Application ELISA
Reactivity Human
SDS SDS - EK1272
Assay Type Sandwich ELISA
Suitable Sample Type serum, plasma, cell culture supernates
Format 96-well strip plate
Storage 4℃ (unopened)standard stored at -20℃, others stored at 4℃ (opened)
Shipping Condition 4℃
Sample Volume 50 μl
Sensitivity 0.72 pg/ml
Standard Curve Range 7.81 - 500 pg/ml
Spike Recovery Range 82 % - 102 %
Mean Spike Recovery 91%
CV of Intra plate 3.7 % - 5.0 %
CV of Inter plate 2.8 % - 6.6 %

96-well polystyrene microplate coated with a monoclonal antibody against CD80

Human CD80 Standard, lyophilized

CD80Detect Antibody

Standard Diluent


Assay Buffer (10×)

Substrate (TMB)

Stop Solution

Washing Buffer (20×)

Plate Covers


This assay employs the quantitative sandwich enzyme immunoassay technique for the quantitative detection of human CD80. The Human CD8/B7-1 ELISA is for research use only. Not for diagnostic or therapeutic procedures.

Cluster of differentiation 80 (CD80) is a protein found on dendritic cells, activated B cells and monocytes that provides a costimulatory signal necessary for T cell activation and survival. It is also known as B7-1. The cDNA for B7-1 predicts a type I membrane protein, i.e., one synthesized with a signal peptide that is cleaved upon translocation across the endoplasmic membrane. The protein is predicted to contain 2 extracellular domains structurally similar to those of Ig, a hydrophobic transmembrane region, and a short cytoplasmic domain. The CD80 and CD86 genes encode B7-1 and B7-2, respectively, which are structurally similar members of the immunoglobulin superfamily expressed on a variety of hematopoietic cell types. B7-1 and B7-2 provide a costimulatory signal to T cells by interacting with CD28 and CTLA4.