MULTI SCIENCES
Human BMP-4 ELISA Kit
Human BMP-4 ELISA Kit
SKU:EK1323
Product Details
| Factory Name | MultiSciences |
|---|---|
| Product Code | 70-EK1323-96 |
| CatNum | EK1323-96 |
| Product Name | Human BMP-4 ELISA Kit |
| Customs Name | Human BMP-4 ELISA Kit |
| Product Spec | 96T |
| SalePrice | 3200 |
| Type | ELISA Kit |
| Application | ELISA |
| Reactivity | Human |
| Modality | NoData |
| Assay Type | Sandwich ELISA |
| Suitable Sample Type | serum, plasma, cell culture supernates |
| Format | 96-well strip plate |
| Storage | 4℃ (unopened) standard stored at -20℃, others stored at 4℃ (opened) |
| Shipping Condition | 4℃ |
| Sensitivity | 0.30 pg/ml |
| Standard Curve Range | 3.91 - 250 pg/ml |
| Spike Recovery Range | 76%-103% |
| Mean Spike Recovery | 92% |
| CV of Intra plate | 1.3 % - 3.5 % |
| CV of Inter plate | 4.9 % - 8.7 % |
| Components |
96-well polystyrene microplate (12 strips of 8 wells) coated with an antibody against human BMP-4 Human BMP-4 Standard, lyophilized BMP-4 Detect Antibody Standard Diluent Streptavidin-HRP Assay Buffer (10×) Substrate Stop Solution Washing Buffer (20×) Adhesive Films |
| PRINCIPLE OF THE ASSAY | Human BMP-4 ELISA Kit is based on the quantitative sandwich enzyme-linked immunosorbent assay technique to measure concentration of human BMP-4 in the samples. An antibody specific for human BMP-4 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and BMP-4 present is bound by the immobilized antibody. After washing away any unbound substances, a biotin-linked detect antibody specific for BMP-4 is added to the wells. Following a wash to remove any unbound antibody-biotin reagent, streptavidin-HRP is added. After washing, substrate solution is added to the wells and color develops in proportion to the amount of BMP-4 bound in the initial step. The color development is stopped and the intensity of the color is measured. |
| Describtion | BMPs are secreted signaling molecules that comprise a subfamily of the TGF-beta superfamily and were originally identified as regulators of cartilage and bone formation. In general, BMPs are implicated in embryogenesis and morphogenesis of various tissues and organs. BMP-4, also known as Bone morphogenetic protein 4, forms disulfide-linked homodimer. It plays an important role in the onset of endochondral bone formation, dorsal/ventral patterning and has also been implicated in the commitment of embryonic mesodermal cells to a hematopoietic fate in a number of systems. It also enhances the formation of adrenergic sympathetic neurons in cultures of neural crest cells. |
