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Human BMP-4 ELISA Kit

Human BMP-4 ELISA Kit


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Human BMP-4 ELISA Kit

Factory Name MultiSciences
Product Code 70-EK1323-96
CatNum EK1323-96
Product Name Human BMP-4 ELISA Kit
Customs Name Human BMP-4 ELISA Kit
Product Spec 96T
SalePrice 3200
Type ELISA Kit
Application ELISA
Reactivity Human
Modality NoData
Assay Type Sandwich ELISA
Suitable Sample Type serum, plasma, cell culture supernates
Format 96-well strip plate
Storage 4℃ (unopened) standard stored at -20℃, others stored at 4℃ (opened)
Shipping Condition 4℃
Sensitivity 0.30 pg/ml
Standard Curve Range 3.91 - 250 pg/ml
Spike Recovery Range 76%-103%
Mean Spike Recovery 92%
CV of Intra plate 1.3 % - 3.5 %
CV of Inter plate 4.9 % - 8.7 %

96-well polystyrene microplate (12 strips of 8 wells) coated with an antibody against human BMP-4

Human BMP-4 Standard, lyophilized

BMP-4 Detect Antibody

Standard Diluent


Assay Buffer (10×)


Stop Solution

Washing Buffer (20×)

Adhesive Films

PRINCIPLE OF THE ASSAY Human BMP-4 ELISA Kit is based on the quantitative sandwich enzyme-linked immunosorbent assay technique to measure concentration of human BMP-4 in the samples. An antibody specific for human BMP-4 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and BMP-4 present is bound by the immobilized antibody. After washing away any unbound substances, a biotin-linked detect antibody specific for BMP-4 is added to the wells. Following a wash to remove any unbound antibody-biotin reagent, streptavidin-HRP is added. After washing, substrate solution is added to the wells and color develops in proportion to the amount of BMP-4 bound in the initial step. The color development is stopped and the intensity of the color is measured.

BMPs are secreted signaling molecules that comprise a subfamily of the TGF-beta superfamily and were originally identified as regulators of cartilage and bone formation. In general, BMPs are implicated in embryogenesis and morphogenesis of various tissues and organs. BMP-4, also known as Bone morphogenetic protein 4, forms disulfide-linked homodimer. It plays an important role in the onset of endochondral bone formation, dorsal/ventral patterning and has also been implicated in the commitment of embryonic mesodermal cells to a hematopoietic fate in a number of systems. It also enhances the formation of adrenergic sympathetic neurons in cultures of neural crest cells.