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Human CA125/MUC16 ELISA Kit

Human CA125/MUC16 ELISA Kit


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Product Details

Human CA125/MUC16 ELISA Kit

Factory Name MultiSciences
Product Code 70-EK1274-96
CatNum EK1274-96
Product Name Human CA125/MUC16 ELISA Kit
Customs Name Human CA125/MUC16 ELISA Kit
Product Spec 96 T
Type ELISA Kit
Application ELISA
Reactivity Human
SDS SDS - EK1274
Assay Type Sandwich ELISA
Suitable Sample Type serum, plasma, cell culture supernates
Format 96-well strip plate
Storage 4℃ (unopened)standard stored at -20℃, others stored at 4℃ (opened)
Shipping Condition 4℃
Sample Volume 50 μl
Sensitivity 0.41 pg/ml
Standard Curve Range 15.63 - 1000  pg/ml
Spike Recovery Range 92 % - 117 %
Mean Spike Recovery 105%
CV of Intra plate 3.2 % - 4.1 %
CV of Inter plate 1.7 % - 2.7 %

96-well polystyrene microplate coated with a monoclonal antibody against CA125

Human CA125 Standard, lyophilized

CA125 Detect Antibody

Standard Diluent


Assay Buffer (10×)

Substrate (TMB)

Stop Solution

Washing Buffer (20×)

Plate Covers


This assay employs the quantitative sandwich enzyme immunoassay technique for the quantitative detection of human CA125/MUC16. The Human CA125/MUC16 ELISA is for research use only. Not for diagnostic or therapeutic procedures.

Cancer antigen 125, carcinoma antigen 125, or carbohydrate antigen 125(CA125) also known as mucin 16 or MUC16, is a protein that in humans is encoded by the MUC16 gene. MUC16 is a member of the mucin family glycoproteins. It is a component of the ocular surface, the respiratory tract and the female reproductive tract epithelia. Since MUC16 is highly glycosylated it creates a hydrophilic environment that acts as a lubricating barrier against foreign particles and infectious agents on the apical membrane of epithelial cells. Also, the cytoplasmic tail of MUC16 has been shown to interact with cytoskeleton by binding members of the ERM protein family. The expression of mucin 16 has been shown to be altered in dry eye, cystic fibrosis, and several types of cancers.