MULTI SCIENCES
Human Calcitonin ELISA Kit
Human Calcitonin ELISA Kit
SKU:EK1301
Product Details
| Factory Name | MultiSciences |
|---|---|
| Product Code | 70-EK1301 - 96 |
| CatNum | EK1301 - 96 |
| Product Name | Human Calcitonin ELISA Kit |
| Customs Name | Human Calcitonin ELISA Kit |
| Product Spec | 96 T |
| Type | ELISA Kit |
| Application | ELISA |
| Reactivity | Human |
| SDS | SDS - EK1301 |
| Assay Type | Sandwich ELISA |
| Suitable Sample Type | serum, plasma, cell culture supernates |
| Format | 96-well strip plate |
| Storage | 4℃ (unopened)standard stored at -20℃, others stored at 4℃ (opened) |
| Shipping Condition | 4℃ |
| Sample Volume | 50ul |
| Sensitivity | 0.50 ng/ml |
| Standard Curve Range | 1.56 - 100pg/ml |
| Spike Recovery Range | 81 % - 106 % |
| Mean Spike Recovery | 92% |
| CV of Intra plate | 1.9% - 3.2% |
| CV of Inter plate | 2.0% - 3.3% |
| Components |
96-well polystyrene microplate coated with a monoclonal antibody against Calcitonin Human Calcitonin Standard, lyophilized Calcitonin Detect Antibody Standard Diluent Assay Buffer (10×) Substrate (TMB) Stop Solution Washing Buffer (20×) Plate Covers |
| Describtion | This assay employs the quantitative sandwich enzyme immunoassay technique. A monoclonal antibody specific for human Calcitonin has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and Calcitonin present is bound by the immobilized antibody. After washing away any unbound substances, a biotin-linked detect antibody specific for Calcitonin is added to the wells. Following a wash to remove any unbound antibody-biotin reagent, Streptavidin-HRP is added. After washing, substrate solution is added to the wells and color develops in proportion to the amount of Calcitonin bound in the initial step. The color development is stopped and the intensity of the color is measured. |
