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Human Calcitonin ELISA Kit

Human Calcitonin ELISA Kit


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Product Details

Human Calcitonin ELISA Kit

Factory Name MultiSciences
Product Code 70-EK1301 - 96
CatNum EK1301 - 96
Product Name Human Calcitonin ELISA Kit
Customs Name Human Calcitonin ELISA Kit
Product Spec 96 T
Type ELISA Kit
Application ELISA
Reactivity Human
SDS SDS - EK1301
Assay Type Sandwich ELISA
Suitable Sample Type serum, plasma, cell culture supernates
Format 96-well strip plate
Storage 4℃ (unopened)standard stored at -20℃, others stored at 4℃ (opened)
Shipping Condition 4℃
Sample Volume 50ul
Sensitivity 0.50 ng/ml 
Standard Curve Range 1.56 - 100pg/ml
Spike Recovery Range 81 % - 106 %
Mean Spike Recovery 92%
CV of Intra plate 1.9% - 3.2%
CV of Inter plate 2.0% - 3.3%

96-well polystyrene microplate coated with a monoclonal antibody against Calcitonin

Human Calcitonin Standard, lyophilized

Calcitonin Detect Antibody

Standard Diluent

Assay Buffer (10×)

Substrate (TMB)

Stop Solution

Washing Buffer (20×)

Plate Covers


This assay employs the quantitative sandwich enzyme immunoassay technique. A monoclonal antibody specific for human Calcitonin has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and Calcitonin present is bound by the immobilized antibody. After washing away any unbound substances, a biotin-linked detect antibody specific for Calcitonin is added to the wells. Following a wash to remove any unbound antibody-biotin reagent, Streptavidin-HRP is added. After washing, substrate solution is added to the wells and color develops in proportion to the amount of Calcitonin bound in the initial step. The color development is stopped and the intensity of the color is measured.