MULTI SCIENCES
Human CarBonic Anhydrase XIV/CA14 Sandwich ELISA Kit
Human CarBonic Anhydrase XIV/CA14 Sandwich ELISA Kit
SKU:EK1225
Product Details
| Brand | MultiSciences |
|---|---|
| CatNum | 70-EK1225 |
| Product Name | Human Carbonic Anhydrase XIV/CA14 ELISA Kit |
| Customs Name | Human Carbonic Anhydrase XIV/CA14 ELISA Kit |
| Application | ELISA |
| Reactivity | Human |
| Assay Type | Sandwich ELISA |
| Suitable Sample Type | serum, plasma, cell culture supernates |
| Format | 96-well strip plate |
| Storage | 4℃ (unopened) standard stored at -20℃, others stored at 4℃ (opened) |
| Shipping Condition | 4℃ |
| Sample Volume | 50 μl |
| Sensitivity | 6.91 pg/ml |
| Standard Curve Range | 312.50 - 20000 pg/ml |
| Spike Recovery Range | 70 % - 129 % |
| Mean Spike Recovery | 0.94 |
| CV of Intra plate | 2.5 % - 5.4 % |
| CV of Inter plate | 3.9 % - 4.5 % |
| Components | 96-well polystyrene microplate coated with a monoclonal antibody against CA14 Human CA14 Standard, lyophilized CA14 Detect Antibody Standard Diluent Assay Buffer (10×) Substrate (TMB) Stop Solution Washing Buffer (20×) Plate Covers |
| Describtion | This assay employs the quantitative sandwich enzyme immunoassay technique for the quantitative detection of human CA14. The Human Carbonic Anhydrase XIV/CA14 ELISA is for research use only. Not for diagnostic or therapeutic procedures. Carbonic anhydrases (CAs) are a large family of zinc metalloenzymes that catalyze the reversible hydration of carbon dioxide. They participate in a variety of biological processes, including respiration, calcification, acid-base balance, bone resorption, and the formation of aqueous humor, cerebrospinal fluid, saliva, and gastric acid. They show extensive diversity in tissue distribution and in their subcellular localization. Carbonic anhydrases XIV (CA14) is a polypeptide consisting of an extracellular N-terminal catalytic domain, a membrane-spanning segment and a short intracellular C-terminal segment with several potential phosphorylation sites. It is expressed in the brain on plasma membranes of neuronal bodies and axons, in the liver on the plasma membranes of hepatocytes, and in the kidney preferentially on the apical membranes of proximal tubules. CA14 shares highest sequence similarity with the other transmembrane CA isoform, CA 12. However, they have different patterns of tissue-specific expression and thus may play different physiologic roles. CA14, together with CA4, play important roles in the regulation of intracellular pH in hippocampal neurons by facilitating AE3-mediated Cl–HCO3-exchange. |
