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Human CCL16/HCC-4/NCC-4 ELISA Kit

Human CCL16/HCC-4/NCC-4 ELISA Kit


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Product Details

Human CCL16/HCC-4/NCC-4 ELISA Kit

Factory Name MultiSciences
Product Code 70-EK1288 - 96
CatNum EK1288 - 96
Product Name Human CCL16/HCC-4/NCC-4 ELISA Kit
Customs Name Human CCL16/HCC-4/NCC-4 ELISA Kit
Product Spec 96 T
Type ELISA Kit
Application ELISA
Reactivity Human
SDS SDS - EK1288
Assay Type Sandwich ELISA
Suitable Sample Type serum, plasma, cell culture supernates
Format 96-well strip plate
Storage 4℃ (unopened)standard stored at -20℃, others stored at 4℃ (opened)
Shipping Condition 4℃
Sample Volume 10ul
Sensitivity 1.38 pg/ml 
Standard Curve Range 7.81-500 pg/ml
Spike Recovery Range
Mean Spike Recovery
CV of Intra plate 2.5% - 4.8%
CV of Inter plate 2.5% - 4.8%

96-well polystyrene microplate coated with a monoclonal antibody against CCL16

Human CCL16 Standard, lyophilized

CCL16 Detect Antibody

Standard Diluent

Assay Buffer (10×)

Substrate (TMB)

Stop Solution

Washing Buffer (20×)

Plate Covers


This assay employs the quantitative sandwich enzyme immunoassay technique. A monoclonal antibody specific for human CCL16 has been pre-coated onto a microplate. Standard, samples and biotin-linked detect antibody specific for CCL16 are pipetted into the wells and CCL16 present is bound by the immobilized antibody and detect antibody following incubation. After washing away any unbound substances, Streptavidin-HRP is added. After washing, substrate solution is added to the wells and color develops in proportion to the amount of CCL16 bound in the initial step. The color development is stopped and the intensity of the color is measured.