MULTI SCIENCES
Human CCL27/CTACK Protein A ELISA Kit
Human CCL27/CTACK Protein A ELISA Kit
SKU:EK1214
Product Details
| Brand | MultiSciences |
|---|---|
| CatNum | 70-EK1214 |
| Product Name | Human CCL27/CTACK ELISA Kit |
| Customs Name | Human CCL27/CTACK ELISA Kit |
| Application | ELISA |
| Reactivity | Human |
| Assay Type | Sandwich ELISA |
| Suitable Sample Type | serum, plasma, cell culture supernates |
| Format | 96-well strip plate |
| Storage | 4℃ (unopened) standard stored at -20℃, others stored at 4℃ (opened) |
| Shipping Condition | 4℃ |
| Sample Volume | 50 μl |
| Sensitivity | 1.85 pg/ml |
| Standard Curve Range | 93.75 - 6000 pg/ml |
| Spike Recovery Range | 86 % - 108 % |
| Mean Spike Recovery | 0.92 |
| CV of Intra plate | 4.4 % - 5.2 % |
| CV of Inter plate | 2.2 % - 2.6 % |
| Components | 96-well polystyrene microplate coated with a monoclonal antibody against CCL27 Human CCL27 Standard, lyophilized CCL27 Detect Antibody Standard Diluent Streptavidin-HRP Assay Buffer (10×) Substrate (TMB) Stop Solution Washing Buffer (20×) Plate Covers |
| Describtion | This assay employs the quantitative sandwich enzyme immunoassay technique for the quantitative detection of human CCL27. The Human CCL27/CTACK ELISA is for research use only. Not for diagnostic or therapeutic procedures. Chemokine (C-C motif) ligand 27 (CCL27), also known as cutaneous T-cell-attracting chemokine (CTACK), is a small cytokine belonging to the CC chemokine family. Its gene is located on human chromosome 9. CCL27 is expressed in numerous tissues, including gonads, thymus, placenta and skin. In vitro expression can be induced beyond constitutive levels by stimulation with IL-1β or TNF-α, while gluticosteroids can suppress it. CCL27 elicits its chemotactic effects by binding to the chemokine receptor CCR10. It is associated with homing of memory T lymphocytes to the skin, and plays a role in T cell-mediated inflammation of the skin. In healthy mice, lymphocytes accumulate at sites of CCL27 injection. In a mouse contact-hypersensitivity model, lymphocyte recruitment is impaired by administration of anti-CCL27 neutralizing antibodies. |
