MULTI SCIENCES
Human CCL2MCP-1 ELISA Kit
Human CCL2MCP-1 ELISA Kit
SKU:EK187
Product Details
| Product Code | 70-EK187-96 |
|---|---|
| CatNum | EK187-96 |
| Product Name | Human CCL2/MCP-1 ELISA Kit |
| Customs Name | Human CCL2/MCP-1 ELISA Kit |
| Product Spec | 96 T |
| Type | ELISA Kit |
| Application | ELISA |
| Reactivity | Human |
| SDS | SDS - EK187 |
| Assay Type | Sandwich ELISA |
| Suitable Sample Type | serum, plasma, cell culture supernates |
| Format | 96-well strip plate |
| Storage | 4℃ (unopened)standard stored at -20℃, others stored at 4℃ (opened) |
| Shipping Condition | 4℃ |
| Sample Volume | 50ul |
| Sensitivity | 2.18pg/ml |
| Standard Curve Range | 31.25-2000pg/ml |
| Spike Recovery Range | 95%-118% |
| Mean Spike Recovery | 107% |
| CV of Intra plate | 3.8% -8.8 % |
| CV of Inter plate | 5.3 % - 7.1% |
| Components |
96-well polystyrene microplate coated with a monoclonal antibody against CCL2/MCP-1 Human CCL2/MCP-1 Standard, lyophilized CCL2/MCP-1 Detect Antibody Standard Diluent Assay Buffer (10×) Substrate (TMB) Stop Solution Washing Buffer (20×) Plate Covers |
| Describtion | This assay employs the quantitative sandwich enzyme immunoassay technique. A monoclonal antibody specific for human CCL2 has been pre-coated onto a microplate. Standard, samples and biotin-linked detect antibody specific for CCL2 are pipetted into the wells and CCL2 present is bound by the immobilized antibody and detect antibody following incubation. After washing away any unbound substances, streptavidin-HRP is added. After washing, substrate solution is added to the wells and color develops in proportion to the amount of CCL2 bound in the initial step. The color development is stopped and the intensity of the color is measured. |
