MULTI SCIENCES
Human CCL4 (MIP-1 Beta) Assay ELISA Kit
Human CCL4 (MIP-1 Beta) Assay ELISA Kit
SKU:EK162
Product Details
| Brand | MultiSciences |
|---|---|
| CatNum | 70-EK162 |
| Product Name | Human CCL4 (MIP-1 beta) ELISA Kit |
| Customs Name | Human CCL4 (MIP-1 beta) ELISA Kit |
| Application | ELISA |
| Reactivity | Human |
| Assay Type | Sandwich ELISA |
| Suitable Sample Type | serum, plasma, cell culture supernates |
| Format | 96-well strip plate |
| Storage | 4℃ (unopened) standard stored at -20℃, others stored at 4℃ (opened) |
| Shipping Condition | 4℃ |
| Sample Volume | 20 μl |
| Sensitivity | 3.58 pg/ml |
| Standard Curve Range | 15.63 - 1000 pg/ml |
| Spike Recovery Range | 85 % - 122 % |
| Mean Spike Recovery | 0.99 |
| CV of Intra plate | 1.8 % - 3.3 % |
| CV of Inter plate | 2.9 % - 3.1 % |
| Components | 96-well polystyrene microplate coated with a monoclonal antibody against CCL4 Human CCL4 Standard, lyophilized CCL4 Detect Antibody Standard Diluent Streptavidin-HRP Assay Buffer (10×) Substrate (TMB) Stop Solution washing Buffer (20×) Plate Covers |
| Describtion | This assay employs the quantitative sandwich enzyme immunoassay technique for the quantitative detection of human CCL4/MIP-1β. The Human CCL4/MIP-1β ELISA is for research use only. Not for diagnostic or therapeutic procedures. Macrophage Inflammatory Proteins (MIP) belong to the family of chemotactic cytokines known as chemokines. In humans, there are two major forms, MIP-1α and MIP-1β that are now officially named CCL3 and CCL4, respectively. CCL4, also known as Macrophage inflammatory protein-1β (MIP-1β) is a CC chemokine with specificity for CCR5 receptors. It is a chemoattractant for natural killer cells, monocytes and a variety of other immune cells. CCL4 is a major HIV-suppressive factor produced by CD8+ T cells.Perforin-low memory CD8+ T cells that normally synthesize CCL4. The cDNAs for human CCL4 encode precursor proteins with a 23 amino acid residue signal peptide that is cleaved to generate the 69 amino acid residue, non-glycosylated mature protein. CCL4 was reported to be twenty-fold less active than CCL3 on the same cell populations. Chemokine activities are mediated by G-protein-coupled seven transmembrane domain receptors. The β chemokine receptor designated CC chemokine receptor-1 (CC CKR-1), alternately named CCL3/RANTES receptor, has been shown to bind CCL3, RANTES, CCL4 and MCP-1 with varying affinities. |
